The Powdery Mildew Effector CSEP0027 Interacts With Barley Catalase to Regulate Host Immunity

文献类型： 外文期刊

第一作者： Yuan, Hongbo

作者： Yuan, Hongbo;Jin, Cong;Pei, Hongcui;Zhao, Lifang;Li, Xue;Li, Jiali;Huang, Wanting;Fan, Renchun;Shen, Qian-Hua;Yuan, Hongbo;Pei, Hongcui;Zhao, Lifang;Li, Xue;Li, Jiali;Fan, Renchun;Shen, Qian-Hua;Huang, Wanting;Liu, Wende

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关键词： powdery mildew; Blumeria graminis; effector; CSEP; virulence; barley catalase

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Powdery mildew is one of the most important fungal pathogen diseases. The genome of barley mildew fungus, Blumeria graminis f. sp. hordei (Bgh), encodes a large number of candidate secreted effector proteins (CSEPs). So far, the function and mechanism of most CSEPs remain largely unknown. Here, we identify a Bgh effector CSEP0027, a member of family 41, triggering cell death in Nicotiana benthamiana. CSEP0027 contains a functional signal peptide (SP), verified by yeast secretion assay. We show that CSEP0027 promotes Bgh virulence in barley infection using transient gene expression and host-induced gene silencing (HIGS). Barley catalase HvCAT1 is identified as a CSEP0027 interactor by yeast two-hybrid (Y2H) screening, and the interaction is verified in yeast, in vitro and in vivo. The coexpression of CSEP0027 and HvCAT1 in barley cells results in altered localization of HvCAT1 from the peroxisome to the nucleus. Barley stripe mosaic virus (BSMV)-silencing and transiently-induced gene silencing (TIGS) assays reveal that HvCAT1 is required for barley immunity against Bgh. We propose that CSEP0027 interacts with barley HvCAT1 to regulate the host immunity and likely reactive oxygen species (ROS) homeostasis to promote fungal virulence during barley infection.

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