Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116

文献类型： 外文期刊

第一作者： Zhu, Jinyu

作者： Zhu, Jinyu;Shi, Xianming;Tan, Zhongfang;Zhang, Zhong

作者机构：

关键词： Exopolysaccharide; Lactobacillus curvatus; Antioxidation activity; Antibacterial activity; Anti-biofilm formation activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 210 卷

页码：

收录情况： SCI

摘要： This study aimed to investigate the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116 from the fish Gymnocypris przewalskii. The purified EPS, denoted as EPS-1, was mainly composed of glucose and mannose at a relative molar ratio of 1:1.05 with molecular weight of 31.9 kDa. The chemical structure of EPS-1 was consisted of -> 2)-alpha-D-Manp-(1 ->, -> 4)-alpha-D-Manp-(1 ->, -> 3,6)-alpha-D-Manp-(1 ->, T beta-D-Glcp-(1 ->, -> 6)-beta-D-Glcp-(1 ->, and -> 3)-beta-D-Glcp-(1 -> glycosidic bonds. A sheet-like structure of dried EPS-1 was determined by scanning electron microscope (SEM), whilst a peak-shaped structure of EPS-1 was observed by atomic force microscope (AFM). The degradation temperature of EPS-1 was determined as 300.21 degrees C using thermogravimetric analysis (TGA). Moreover, the antioxidant capacity of EPS-1 at a concentration of 5.0 mg/mL against DPPH and ABTS was 84.50% and 92.53%, respectively. Furthermore, EPS-1 exhibited acceptable bacteriostatic efficacy against S. Enteritidis, E. coli, and S.aureus with significant inhibition of S. Enteritidis biofilm formation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序