Dual-electrode signal amplification self-powered biosensing platform based on nanozyme boosting target-induced DNA nanospace array for ultrasensitive detection of sugarcane Pokkah Boeng disease pathogenic bacteria

文献类型： 外文期刊

第一作者： Song, Yujie

作者： Song, Yujie;Luo, Hu;Huang, Ke-Jing;Tan, Xuecai;Wang, Zeping;Liao, Jie;Zhang, Xiaoqiu;Yan, Jun;Ya, Yu

作者机构：

关键词： Sugarcane pokkah boeng disease pathogenic; bacteria; Noble metal nanoparticles/metal oxide; nanozymes; Dual-electrode signal amplification

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2024 年 281 卷

页码：

收录情况： SCI

摘要： Sugarcane is a crop with significant economic importance worldwide. However, pokkah boeng disease poses a serious threat to its production and the sustainable development. There is a pressing necessity for precise and portable detection methods. We develop a dual-electrode signal amplification biosensing platform, for highly sensitive detection of sugarcane pokkah boeng disease pathogenic bacteria. This innovative platform integrates highly catalytic AuNPs/Mn3O4 nanozymes with N-GDY, along with a target-induced development of DNA nanostructure arrays. AuNPs/N-GDY serves as dual electrode substrates, and AuNPs/Mn3O4 nanozymes are surface-loaded as the bioanode. The biocathode is constructed by introducing DNA nanospace arrays onto the electrode through target-induced methods. [Ru(NH3)6]3+ is embedded into the nucleic acid double-helix scaffold via electrostatic adsorption, generating an EOCV signal that is strongly correlated with the target concentration. To further enhance sensitivity, the detection platform is combined with a capacitor to amplify the detection signal, utilizing its high power density, which results in a 22.5-fold increase in sensitivity. The method offers a linear detection range of 0.0001 to 10,000 pM and an detection limit of 32.5 aM (S/N = 3). This method supplies a novel approach for real-time monitoring and competent oversight of pokkah boeng disease pathogenic bacteria.

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