Molecular Characterization, Evolution and Expression Analysis of Ammonium Transporter from Four Closely Related Bactrocera Species (Tephritidae)

文献类型： 外文期刊

第一作者： Zhang, Jie

作者： Zhang, Jie;Wang, Qi;Liu, Chenhao;Qian, Qian;Ru, Chuanjian;Yan, Shanchun;Liu, Jiaying;Liu, Leyuan;Liu, Wei;Wang, Guirong

作者机构：

关键词： Ammonium Transporter; olfactory receptor; Tephritidae

期刊名称：LIFE-BASEL （ 影响因子：3.4； 五年影响因子：3.4 ）

ISSN：

年卷期： 2024 年 14 卷 9 期

页码：

收录情况： SCI

摘要： Numerous insects are attracted to low levels of ammonia, utilizing it as a cue to locate food sources. The Ammonium Transporter (Amt), a highly conserved, atypical olfactory receptor, has been shown to mediate the detection of ammonia in insects. While the attraction of Tephritidae to ammonia is well established, knowledge about the Amt in this family is limited. The species Bactrocera dorsalis (Hendel 1912), Bactrocera cucurbitae (Coquillett 1899), Bactrocera correcta Bezzi 1916 and Bactrocera tau (Walker 1849), which are common agricultural pests within Tephritidae, exhibit numerous ecological similarities, offering a solid foundation for studying Amt characteristics in this family. In this study, we elucidated the sequences, evolutionary relationships, and expression patterns of Amt in these four species. The results indicated that these Amts share the same open reading frame, containing 1770 bp that encode a protein of 589 amino acid residues. These Amt proteins exhibit the typical structural characteristics of Amts, including an 11-transmembrane domain with an extracellular N-terminus and an intracellular C-terminus. They also have the ability to form trimers in the membrane. Additionally, they contain three conserved amino acid residues essential for ammonia transport: A189, H195, and H352. Phylogenetic and expression pattern analyses showed that they are highly conserved in Diptera and are significantly expressed in antennae. This study is the first report characterizing the Amt gene in four Tephritidae species. These findings provide a foundation for further exploration into the roles of these genes in their particular biological contexts.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序