Epmedin C Alleviates Deoxynivalenol-Induced Immunotoxicity by Inhibiting Caspase-1 Activation in Chicken Macrophages

文献类型： 外文期刊

第一作者： Bu, Wandi

作者： Bu, Wandi;Wu, Xinyao;Zhong, Zhihang;Li, Yuekai;Fang, Yaping;Chen, Shuang;Wang, Zige;Hong, Ying;Jiang, Jun;Deng, Yiqun;Bu, Wandi;Chen, Shuang;Deng, Yiqun;Bu, Wandi;Wu, Xinyao;Zhong, Zhihang;Li, Yuekai;Fang, Yaping;Chen, Shuang;Wang, Zige;Hong, Ying;Dai, Feiyang;Jiang, Jun;Wang, Yan

作者机构：

关键词： deoxynivalenol; chickens; caspase-1; macrophage; epmedin C

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON), a prevalent mycotoxin in poultry feed, exerts immunotoxicity even at low doses, yet its mechanisms in poultry remain unclear. Here, we evaluated DON's effects using chicken macrophage HD11 cells and one-day-old chicks. In vitro, DON activated the caspase-1/IL-1 beta pathway, increased reactive oxygen species (ROS), and promoted proinflammatory cytokine release, impairing antibody production. Network pharmacology and molecular docking identified epmedin C, a major flavonoid from Epimedium, as a potential caspase-1 inhibitor. Epmedin C bound strongly to caspase-1, inhibited its activation, reduced ROS, and suppressed cytokine secretion in HD11 cells, while coculture assays confirmed restoration of antibody production. In vivo, dietary epmedin C supplementation alleviated DON-induced immunotoxicity by restoring antibody levels, reducing splenic caspase-1 activity, and preserving immune and intestinal structures. These findings highlight epmedin C as a natural caspase-1 inhibitor that mitigates DON-induced immune damage, offering a promising strategy for mycotoxin detoxification in poultry.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序