Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana

文献类型： 外文期刊

第一作者： Dong, Na

作者： Dong, Na;Liu, Xin;Lu, Yan;Du, LiPu;Xu, Huijun;Liu, Hongxia;Xin, Zhiyong;Zhang, Zengyan

作者机构：

关键词： Bipolaris sorokiniana;Disease resistance;Ethylene-responsive factor (ERF);Transgenic wheat;Triticum aestivum

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Bipolaris sorokiniana is an economically important phytopathogen of wheat and other cereal species. In this paper, a novel pathogen-induced ethylene-responsive factor (ERF) gene of wheat, TaPIEP1, was isolated and characterized. The transcript of TaPIEP1 was significantly and rapidly induced by treatments with B. sorokiniana, and with ethylene (ET), jasmonate (JA), and abscisic acid. Molecular and biochemical assays demonstrated that TaPIEP1 is a new ERF transcription activator belonging to B-3c subgroup of the ERF family. Transgenic wheat lines overexpressing TaPIEP1 were generated by biolistic bombardment and molecular screening. Compared with the host wheat Yangmai12, six stable transgenic wheat lines overexpressing TaPIEP1 that exhibited significantly increased resistance to B. sorokiniana were identified by molecular detection in the T_0-T_4 generations and by disease resistance tests. The degree of the enhanced resistance was correlated with an accumulation of the transcript level of TaPIEP1. Furthermore, the transcript levels of certain defense-related genes in the ET/JA pathways were markedly increased in the transgenic wheat plants with enhanced resistance. These results reveal that TaPIEP1 overexpression in wheat could obviously improve resistance to B. sorokiniana via activation of some defense genes, and TaPIEP1 gene may be useful in improving crop resistance to the pathogen.

分类号： Q78

  • 相关文献

[1]An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. Liu, Xin,Wang, Xindong,Zhou, Xianyao,Ye, Xingguo,Wei, Xuening,Zhou, Miaoping. 2012

[2]A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses. Saad, Abu Sefyan I.,Li, Xu,Li, He-Ping,Huang, Tao,Gao, Chun-Sheng,Guo, Mao-Wei,Cheng, Wei,Liao, Yu-Cai,Li, He-Ping,Gao, Chun-Sheng,Liao, Yu-Cai,Zhao, Guang-Yao.

[3]Expression of a potato antimicrobial peptide SN1 increases resistance to take-all pathogen Gaeumannomyces graminis var. tritici in transgenic wheat. Rong, Wei,Qi, Lin,Wang, Jingfen,Du, Lipu,Xu, Huijun,Zhang, Zengyan,Rong, Wei,Wang, Aiyun. 2013

[4]Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Li, Zhao,Zhang, Zengyan,Du, Lipu,Xu, Huijun,Xin, Zhiyong,Li, Zhao,Zhou, Miaoping,Ren, Lijuan,Zhang, Boqiao.

[5]Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. Lu, Yuqing,Yao, Miaomiao,Zhang, Jinpeng,Song, Liqiang,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Li, Lihui.

[6]Functional markers in wheat: current status and future prospects. Liu, Yanan,He, Zhonghu,Xia, Xianchun,He, Zhonghu,Appels, Rudi.

[7]Wheat bHLH transcription factor gene, TabHLH060, enhances susceptibility of transgenic Arabidopsis thaliana to Pseudomonas syringae. Lin, Ruiming,Feng, Jing,Qiu, Dewen,Chen, Wanquan,Xu, Shichang.

[8]Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th ponticum. Li, Hongjie,Conner, R. L.,Murray, T. D..

[9]GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat. Wang, Aiyun,Rong, Wei,Wang, Aiyun,Wei, Xuening,Rong, Wei,Dang, Liang,Du, Li-Pu,Qi, Lin,Xu, Hui-Jun,Zhang, Zengyan,Dang, Liang,Shao, Yanjun.

[10]Two members of TaRLK family confer powdery mildew resistance in common wheat. Tingting Chen,Jin Xiao,Jun Xu,Wentao Wan,Bi Qin,Aizhong Cao,Wei Chen,Liping Xing,Chen Du,Xiquan Gao,Shouzhong Zhang,Ruiqi Zhang,Wenbiao Shen,Haiyan Wang,Xiue Wang. 2016

[11]RNA-seq analysis of unintended effects in transgenic wheat overexpressing the transcription factor GmDREB1. Jiang, Qiyan,Niu, Fengjuan,Sun, Xianjun,Hu, Zheng,Li, Xinhai,Ma, Youzhi,Zhang, Hui. 2017

[12]Expression of the intact C-4 type pepc gene cloned from maize in transgenic winter wheat. Chen, XQ,Zhang, XD,Liang, RQ,Zhang, LQ,Yang, FP,Cao, MQ. 2004

[13]Improvement of the photosynthetic characteristics of transgenic wheat plants by transformation with the maize C4 phosphoenolpyruvate carboxylase gene. Hu, Lin,Li, Yan,Xu, Weigang,Zhang, Qingchen,Zhang, Lei,Qi, Xueli,Dong, Haibin,Hu, Lin. 2012

[14]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[15]Drought tolerance and proteomics studies of transgenic wheat containing the maize C-4 phosphoenolpyruvate carboxylase (PEPC) gene. Qin, Na,Xu, Weigang,Hu, Lin,Li, Yan,Wang, Huiwei,Qi, Xueli,Fang, Yuhui,Hua, Xia.

[16]Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene. Chen, Ming,Ma, Youzhi,Du, Lipu,Ye, Xingguo,Pang, Junlan,Zhang, Xinmei,Li, Liancheng,Xu, Huijun,Sun, Liying,Chen, Jiong,Andika, Ida B.,Chen, Jianping.

[17]The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants. Gao, Shi-Qing,Zhao, Chang-Ping,Tang, Yi-miao,Ma, You-Zhi,Zhao, Xin,Gao, Shi-Qing,Chen, Ming,Xu, Zhao-Shi,Li, Liancheng,Xu, Hui-jun,Ma, You-Zhi.

[18]Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat. Zhang, HuiFang,Zhang, HuiFang,Xu, WeiGang,Wang, HuiWei,Hu, Lin,Li, Yan,Qi, XueLi,Zhang, Lei,Li, ChunXin,Hua, Xia.

[19]The overaccumulation of glycinebetaine alleviated damages to PSII of wheat flag leaves under drought and high temperature stress combination. Wang, Gui-Ping,Tian, Feng-Xia,Zhang, Meng,Wang, Wei.

[20]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

作者其他论文 更多>>

微信小程序