TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize

文献类型： 外文期刊

第一作者： Lyu, Mingjie

作者： Lyu, Mingjie;Fu, Junjie;Liu, Jun;Cheng, Xiliu;Liu, Huafeng;Chen, Yanhui;Ku, Lixia;Liu, Huafeng;Chen, Yanhui;Ku, Lixia;Waititu, Joram Kiriga;Sun, Ying;Wang, Huan

作者机构：

关键词： Maize; Zea mays; Insertion identification; Ds transposon; Mutant library; Next-generation sequencing; Functional genomics

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：5.723； 五年影响因子：5.755 ）

ISSN： 1673-8527

年卷期： 2021 年 48 卷 11 期

页码：

收录情况： SCI

摘要： In plants, transposable element (TE)-triggered mutants are important resources for functional genomic studies. However, conventional approaches for genome-wide identification of TE insertion sites are costly and laborious. This study developed a novel, rapid, and high-throughput TE insertion site identification workflow based on next-generation sequencing and named it Transposable Element Amplicon Sequencing (TEAseq). Using TEAseq, we systemically profiled the Dissociation (Ds) insertion sites in 1606 independent Ds insertional mutants in advanced backcross generation using K17 as background. The Ac-containing individuals were excluded for getting rid of the potential somatic insertions. We characterized 35,696 germinal Ds insertions tagging 10,323 genes, representing approximately 23.3% of the total genes in the maize genome. The insertion sites were presented in chromosomal hotspots around the ancestral Ds loci, and insertions occurred preferentially in gene body regions. Furthermore, we mapped a loss-of-function AGL2 gene using bulked segregant RNA-sequencing assay and proved that AGL2 is essential for seed development. We additionally established an open-access database named MEILAM for easy access to Ds insertional mutations. Overall, our results have provided an efficient workflow for TE insertion identification and rich sequence-indexed mutant resources for maize functional genomic studies. Copyright (C) 2021, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.

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