Whole genome sequencing and antimicrobial resistance analysis of Vibrio parahaemolyticus Vp2015094 carrying an antimicrobial-resistant plasmid

文献类型： 外文期刊

第一作者： Wang, Ting

作者： Wang, Ting;Yao, Lin;Qu, Meng;Wang, Lianzhu;Li, Fengling;Tan, Zhijun;Jiang, Yanhua;Wang, Ting;Wang, Peng;Tan, Zhijun;Jiang, Yanhua;Wang, Peng

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关键词： Vibrio parahaemolyticus; Whole genome sequencing; Antimicrobial resistance gene; Plasmid

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：4.349； 五年影响因子：3.915 ）

ISSN： 2213-7165

年卷期： 2022 年 30 卷

页码：

收录情况： SCI

摘要： Objectives: Vibrio parahaemolyticus is an international foodborne pathogen that has caused many human infection cases. The emergence of antimicrobial resistance (AMR) in V. parahaemolyticus strains can hinder the curative effect of antimicrobials. The aim of this study was therefore to investigate the whole genome sequence of V. parahaemolyticus Vp2015094 from seafood and to explore the genetic mechanisms of AMR. Methods: The whole genome sequencing was performed using PacBio combined with Illumina platform. The antimicrobial resistance genes (ARGs) were detected by Resistance Gene Identifier in the Comprehensive Antibiotic Resistance Database. The antimicrobial-resistant plasmid of Vp2015094 was compared with other similar plasmids from different sources. Results: Vibrio parahaemolyticus Vp2015094 contained two chromosomes and two plasmids. ARGs were identified on chromosome and plasmid pVp94-1. Plasmid pVp94-1 carried tetracycline resistance genes (tetB, tetM, tetR, tetC), aminoglycoside resistance genes (aph(3'')-Ib, aph(6)-Id), sulphonamide resistance genes (sul2), diaminopyrimidine resistance gene (dfrA6), fluoroquinolone resistance gene (qnrVC6), phenicol resistance gene (floR) and penam resistance gene (blaCARB-19), which were surrounded by transposase genes. Plasmid pVp94-1 was a conjugative plasmid with a high transfer frequency. Conclusion: Vibrio parahaemolyticus Vp2015094 exhibited multi-antimicrobial resistance that was mediated by chromosome and plasmid. Plasmid and transposon were speculated to be responsible for the dissemination of ARGs. This study provided an understanding of the genetic mechanisms of AMR of V. parahaemolyticus from seafood, which needs continued monitoring. (C) 2022 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.

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