LncRNA SMARCD3-OT1 Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation via Enhancing SMARCD3X4 Expression

文献类型： 外文期刊

第一作者： Zhang, Jing

作者： Zhang, Jing;Cai, Bolin;Ma, Manting;Kong, Shaofen;Zhou, Zhen;Zhang, Xiquan;Nie, Qinghua;Zhang, Jing;Cai, Bolin;Ma, Manting;Kong, Shaofen;Zhou, Zhen;Zhang, Xiquan;Nie, Qinghua;Zhang, Jing;Cai, Bolin;Ma, Manting;Kong, Shaofen;Zhou, Zhen;Zhang, Xiquan;Nie, Qinghua;Zhang, Jing;Cai, Bolin;Ma, Manting;Kong, Shaofen;Zhou, Zhen;Zhang, Xiquan;Nie, Qinghua

作者机构：

关键词： lncRNA; myogenesis; myoblast; cell proliferation; cell differentiation; CDKN1A; MYOD; SMARCD3

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 9 期

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收录情况： SCI

摘要： Long noncoding RNA (lncRNA) plays a crucial part in all kinds of life activities, especially in myogenesis. SMARCD3 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily d, member 3) is a member of the SWI/SNF protein complex and was reported to be required for cell proliferation and myoblast differentiation. In this study, we identified a new lncRNA named SMARCD3-OT1 (SMARCD3 overlapping lncRNA), which strongly regulated the development of myogenesis by improving the expression of SMARCD3X4 (SMARCD3 transcripts 4). We overexpressed and knockdown the expression of SMARCD3-OT1 and SMARCD3X4 to investigate their function on myoblast proliferation and differentiation. Cell experiments proved that SMARCD3-OT1 and SMARCD3X4 promoted myoblast proliferation through the CDKN1A pathway and improved differentiation of differentiated myoblasts through the MYOD pathway. Moreover, they upregulated the fast-twitch fiber-related genes and downregulated the slow-twitch fiber-related genes, which indicated that they facilitated the slow-twitch fiber to transform into the fast-twitch fiber. The animals' experiments supported the results above, demonstrating that SMARCD3-OT1 could induce muscle hypertrophy and fast-twitch fiber transformation. In conclusion, SMARCD3-OT1 can improve the expression of SMARCD3X4, thus inducing muscle hypertrophy. In addition, SMARCD3-OT1 can facilitate slow-twitch fibers to transform into fast-twitch fibers.

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