An effector CSEP087 from Erysiphe necator targets arginine decarboxylase VviADC to regulate host immunity in grapevine

文献类型： 外文期刊

第一作者： Mu, Bo

作者： Mu, Bo;Chen, Jinfu;Wang, Hongyan;Kong, Wenxiang;Wen, Ying-Qiang;Mu, Bo;Chen, Jinfu;Wang, Hongyan;Kong, Wenxiang;Wen, Ying-Qiang;Fan, Xiucai

作者机构：

关键词： Powdery mildew; Grape; Effector; Hydrogen peroxide; Immune system

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）

ISSN： 0304-4238

年卷期： 2022 年 303 卷

页码：

收录情况： SCI

摘要： Grapevine (Vitis vinifera) is suffering from the fungal pathogen powdery mildew, which affects grape production worldwide. Powdery mildew secretes a large number of effectors into plant cells, which modulate the innate immune system to promote colonization of the infection. Many studies have been conducted on the interactions between pathogens and plants, but the working mechanism of the effectors in the interaction between powdery mildew and grapevine has not been reported. In this study, we identified the CSEP087 effector from the grapevine powdery mildew isolate, Erysiphe necator NAFU1, which was upregulated during infection and contained a signal peptide that was secreted into the plant cells as verified by a yeast secretion assay. The expression of CSEP087 in grapevine leaves facilitated the growth of powdery mildew and suppressed the production of reactive oxygen species (ROS). CSEP087 interacted with VviADC, an arginine decarboxylase from grapevine, as screened by a yeast two-hybrid system. VviADC is a conserved protein in horticultural and model plants, particularly localized at the recognition and catalytic sites of the enzyme active center. The expression of VviADC in grapevine leaves inhibited the growth of powdery mildew and promoted ROS production, including hydrogen peroxide in the plants. In addition, VviADC increases the enzymes related to ROS scavenging activities. Our results demonstrate that the powdery mildew effector CSEP087 targeted VviADC, which is a regulator of hydrogen peroxide production and plant immunity. This finding advances our understanding of the role of effectors in the grapevine-powdery mildew interaction.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序