Screening of Endophytic Fungi in Locoweed Induced by Heavy-Ion Irradiation and Study on Swainsonine Biosynthesis Pathway

文献类型： 外文期刊

第一作者： Mo, Yanan

作者： Mo, Yanan;Yang, Zhen;Hao, Baocheng;Cheng, Feng;Shang, Xiaofei;Zhao, Haoxia;Shang, Ruofeng;Wang, Xuehong;Liang, Jianping;Wang, Shengyi;Liu, Yu;Mo, Yanan;Yang, Zhen;Hao, Baocheng;Cheng, Feng;Shang, Xiaofei;Zhao, Haoxia;Shang, Ruofeng;Wang, Xuehong;Liang, Jianping;Wang, Shengyi;Liu, Yu;Mo, Yanan;Yang, Zhen;Hao, Baocheng;Cheng, Feng;Shang, Xiaofei;Zhao, Haoxia;Shang, Ruofeng;Wang, Xuehong;Liang, Jianping;Wang, Shengyi;Liu, Yu;Cheng, Feng;Song, Xiangdong;Liu, Yu

作者机构：

关键词： Alternaria section Undifilum oxytropis; swainsonine; heavy-ion irradiation; metabonomics; biosynthesis pathway

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 9 期

页码：

收录情况： SCI

摘要： Swainsonine (SW) is a substance with both animal neurotoxicity and natural anticancer activity produced by the metabolism of endophytic fungus Alternaria section Undifilum oxytropis of locoweed. This paper produced SW by fermentation of the endophytic fungus A. oxytropis of locoweed and obtained the optimal ultrasonic-assisted extraction process of SW by the response surface methodology. Meanwhile, four mutant strains with significant and stable SW-producing properties were screened out after the mutagenesis of A. oxytropis by heavy-ion irradiation. Of these, three were high-yielding stains and one was a low-yielding strain. In addition, through the analysis of metabolomics studies, it was speculated that the different SW production performance of the mutant might be related to the biosynthesis and utilization of L-lysine, L-2-aminoadipate-6-semialdehyde, etc. These results laid the foundation for the expansion of SW production, artificial construction of low-toxic locoweed and clarification of the SW biosynthesis pathway in A. oxytropis.

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