Genome-wide identification, evolution and expression analysis of bone morphogenetic protein (BMP) gene family in chinese soft-shell turtle (Pelodiscus sinensis)

文献类型： 外文期刊

第一作者： Lei, Luo

作者： Lei, Luo;Zhu, Junxian;Chen, Chen;Wang, Yongchang;Wu, Congcong;Wang, Yakun;Liu, Xiaoli;Hong, Xiaoyou;Yu, Lingyun;Chen, Haigang;Wei, Chengqing;Liu, Yihui;Li, Wei;Zhu, Xinping;Lei, Luo;Zhu, Xinping;Zhu, Junxian;Zhu, Xinping;Qi, Ming

作者机构： Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Guangzhou, Guangdong, Peoples R China;Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China;Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China;Zhejiang Fisheries Tech Extens Ctr, Hangzhou, Peoples R China

关键词： Pelodiscus sinensis; bone morphogenetic protein; gene family; genome-wide; transcriptome; sex differentiation

期刊名称：FRONTIERS IN GENETICS （ 2022影响因子：3.7； 五年影响因子：4.3 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Introduction: Bone morphogenetic proteins (BMPs) play a crucial role in bone formation and differentiation. Recent RNA-Seq results suggest that BMPs may be involved in the sex differentiation of P. sinensis, yet more relevant studies about BMPs in P. sinensis are lacking. Methods: Herein, we identified BMP gene family members, analyzed the phylogeny, collinear relationship, scaffold localization, gene structures, protein structures, transcription factors and dimorphic expression by using bioinformatic methods based on genomic and transcriptomic data of P. sinensis. Meanwhile, qRT-PCR was used to verify the RNA-Seq results and initially explore the function of the BMPs in the sex differentiation of P. sinensis. Results: A total of 11 BMP genes were identified, 10 of which were localized to their respective genomic scaffolds. Phylogenetic analysis revealed that BMP genes were divided into eight subfamilies and shared similar motifs ("WII", "FPL", "TNHA", "CCVP", and "CGC") and domain (TGF-beta superfamily). The results of the sexually dimorphic expression profile and qRT-PCR showed that Bmp2, Bmp3, Bmp15l, Bmp5, Bmp6 and Bmp8a were significantly upregulated in ovaries, while Bmp2lb, Bmp7, Bmp2bl and Bmp10 were remarkable upregulated in testes, suggesting that these genes may play a role in sex differentiation of P. sinensis. Discussion: Collectively, our comprehensive results enrich the basic date for studying the evolution and functions of BMP genes in P. sinensis.

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