Avian influenza virus and Newcastle disease virus (NDV) surveillance in commercial breeding farm in China and the characterization of Class I NDV isolates

文献类型： 外文期刊

第一作者： Hu, Beixia

作者： Hu, Beixia;Huang, Yanyan;Xu, Chuantian;Zhang, Wei;Yan, Shigan;Zhang, Xiumei;He, Yefeng;Lu, Xishan;Meng, Bin

作者机构：

关键词： nucleotide sequence

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In order to determine the actual prevalence of avian influenza virus (AIV) and Newcastle disease virus (NDV) in ducks in Shandong province of China, extensive surveillance studies were carried out in the breeding ducks of an intensive farm from July 2007 to September 2008. Each month cloacal and tracheal swabs were taken from 30 randomly selected birds that appeared healthy. All of the swabs were negative for influenza A virus recovery, whereas 87.5% of tracheal swabs and 100% cloacal swabs collected in September 2007, were positive for Newcastle disease virus isolation. Several NDV isolates were recovered from tracheal and cloacal swabs of apparently healthy ducks. All of the isolates were apathogenic as determined by the MDT and ICPI. The HN gene and the variable region of F gene (nt 47-420) of four isolates selected at random were sequenced. A 374 bp region of F gene and the full length of HN gene were used for phylogenetic analysis. Four isolates were identified as the same isolate based on nucleotide sequences identities of 99.2-100%, displaying a closer phylogenetic relationship to lentogenic Class I viruses. There were 1.9-9.9% nucleotide differences between the isolates and other Class 1 virus in the variable region of F gene (nt 47-420), whereas there were 38.5-41.2% nucleotide difference between the isolates and Class II viruses. The amino acid sequences of the F protein cleavage sites in these isolates were 112-ERQERL-117. The full length of HN gene of these isolates was 1851 bp, coding 585 amino acids. The homology analysis of the nucleotide sequence of HN gene indicated that there were 2.0-4.2% nucleotide differences between the isolates and other Class II viruses, whereas there were 29.5-40.9% differences between the isolates and Class II viruses. The results shows that these isolates are not phylogenetically related to the vaccine strain (LaSota). This study adds to the understanding of the ecology of influenza viruses and Newcastle disease viruses in ducks and emphasizes the need for constant surveillance in times of an ongoing and expanding epidemic of AIV and NDV

分类号： S8

  • 相关文献

[1]DEVELOPMENT OF MICROSATELLITE MARKERS IN KMERIA SEPTENTRIONALIS (MAGNOLIACEAE), AN ENDANGERED CHINESE TREE. Lin, Yanfang,Zhang, Qiwei,Wang, Yaofang,Tang, Shaoqing,Lin, Yanfang,Tang, Shaoqing,Zeng, Liyan.

[2]Development and characterization of 12 microsatellite markers for Johnius belengerii. Ma, Chunyan,Ma, Hongyu,Ma, Lingbo,Shen, Anglv,Gao, Lujiao.

[3]Myceligenerans halotolerans sp. nov., an actinomycete isolated from a salt lake, and emended description of the genus Myceligenerans. Tang, Shu-Kun,Li, Wen-Jun,Tang, Shu-Kun,Li, Wen-Jun,Wang, Yun,Li, Zhi,Lou, Kai,Li, Zhi,Mao, Pei-Hong,Jin, Xiang,Klenk, Hans-Peter,Zhang, Li-Xin,Li, Wen-Jun.

[4]Complete Genome Sequences of Brucella melitensis Strains M28 and M5-90, with Different Virulence Backgrounds. Bu, Zhigao.

[5]Cloning and Sequence Analysis of Prophenoloxidase from Haemocytes of the Red Swamp Crayfish, Procambarus clarkii. Li Yan-he,Chen Hong-quan,Zheng Fang-liang,Wang Han-zhong,Li Yan-he,Wang Liu-quan,Xu Di-ping. 2009

[6]Sequence of a second isolate of Chinese wheat mosaic furovirus. Yang, J,Chen, J,Chen, J,Jiang, H,Zhao, Q,Adams, MJ. 2001

[7]Genome organization and variation in the 3 '-partial sequence of garlic latent virus in China. Chen, J,Zheng, HY,Chen, JP,Yang, CL. 2002

[8]Molecular analysis of barley yellow mosaic virus isolates from China. Chen, J,Shi, NN,Cheng, Y,Diao, A,Chen, JP,Wilson, TMA,Antoniw, JF,Adams, MJJ. 1999

[9]Complete Genome Sequence of the Nitrogen-Fixing and Rhizosphere-Associated Bacterium Pseudomonas stutzeri Strain DSM4166. Yu, Haiying,Lu, Wei,Dai, Shuxian,Li, Qin,Yang, Zhimin,Deng, Zhiping,Zhang, Wei,Chen, Ming,Ping, Shuzhen,Han, Yunlei,Zhan, Yuhua,Yan, Yongliang,Lin, Min,Yuan, Menglong,Yang, Jian,Dong, Jie,Sun, Lilian,Jin, Qi,Yang, Jian,Dong, Jie,Sun, Lilian,Jin, Qi.

[10]The gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein. Luo, ZL,Chen, XY,Gao, DM,Fang, RX. 1998

[11]Characterization of a New Strain of Capsicum chlorosis virus from Peanut (Arachis hypogaea L.) in China. Chen, K.,Xu, Z.,Yan, L.,Wang, G..

[12]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[13]Sequence diversity in the coat protein coding region of wheat yellow mosaic bymovirus isolates from China. Chen, J,Chen, J,Du, J,Adams, MJ. 2000

[14]Genetic diversity comparison between Chinese and Japanese soybeans (Glycine max (L.) Merr.) revealed by nuclear SSRs. Guan, Rongxia,Chang, Ruzhen,Li, Yinghui,Wang, Lixia,Liu, Zhangxiong,Qiu, Lijuan.

[15]Isolation of serotype 2 porcine teschovirus in China: Evidence of natural recombination. Wang, Bin,Tian, Zhi-Jun,Gong, Da-Qing,Li, Deng-Yun,Wang, Yao,Chen, Jia-Zeng,An, Tong-Qing,Peng, Jin-Mei,Wang, Bin,Tong, Guang-Zhi,Wang, Bin,Chen, Jia-Zeng,Peng, Jin-Mei,Tong, Guang-Zhi.

[16]Complete Genome Sequence of Aeromonas veronii Strain B565. Liu, Yuchun,Zhang, Yuting,Zhou, Zhigang,Li, Yanxia,Zhou, Zhemin,Huang, Huoqing,Ren, Yan,Li, Guannan,Wang, Lei,Zhou, Zhemin,Ren, Yan,Wang, Lei,Ren, Yan,Wang, Lei.

[17]Molecular cloning of prolactin receptor of the Peking duck. Wang, J.,Hou, S. S.,Huang, W.,Yang, X. G.,Zhu, X. Y.,Yang, X. G.,Zhu, X. Y.,Liu, X. L..

[18]Development of eighteen polymorphic microsatellite markers in Scylla paramamosain by 5 ' anchored PCR technique. Cui, Haiyu,Ma, Hongyu,Ma, Lingbo,Ma, Chunyan,Ma, Qunqun,Cui, Haiyu,Ma, Qunqun.

[19]FMD subunit vaccine produced using a silkworm-baculovirus expression system: Protective efficacy against two type Asia1 isolates in cattle. Li, Zhiyong,Yin, Xiangping,Li, Xuerui,Li, Baoyu,Lan, Xi,Liu, Jixing,Yi, Yongzhu,Zhang, Zhifang.

[20]Cloning and characterization of a beta-1,3-glucan-binding protein from shrimp Fenneropenaeus chinensis. Lai, Xiaofang,Kong, Jie,Wang, Qingyin,Wang, Weiji,Meng, Xianhong,Lai, Xiaofang,Lai, Xiaofang.

作者其他论文 更多>>

微信小程序