Comparative analysis of the molecular response characteristics in Platycodon grandiflorus irradiated with heavy ion beams and X-rays

文献类型： 外文期刊

第一作者： Liu, Xiao

作者： Liu, Xiao;Du, Yan;Xu, Chaoli;Li, Xuehu;Wang, Yuanmeng;Ge, Linghui;Ren, Weibin;Zhou, Libin;Liu, Xiao;Du, Yan;Li, Xuehu;Ren, Weibin;Zhou, Libin;Xu, Chaoli;Wang, Fusheng;Liu, Luxiang;Ma, Xiaohui;Wang, Yuanmeng;Ge, Linghui;Jin, Ling;Zhou, Libin;Zhou, Libin

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关键词： Platycodon grandiflorus; Heavy ion beams; X-rays; Transcriptome; Proteome

期刊名称：LIFE SCIENCES IN SPACE RESEARCH （ 影响因子：2.5； 五年影响因子：2.3 ）

ISSN： 2214-5524

年卷期： 2023 年 38 卷

页码：

收录情况： SCI

摘要： The response of plants to radiation is an essential topic in both space plant cultivation and mutation breeding by radiation. In this study, heavy ion beams (HIB) generated by the ground accelerator and X-rays (XR) were used as models of high linear energy transfer (LET) and low LET radiation to study the molecular response mechanism of Platycodon grandiflorus (P. grandiflorus) seedlings after irradiation. The gene and protein expression profiles of P. grandiflorus after 15 Gy HIB and 20 Gy XR radiation were analyzed by transcriptome and proteome. The results showed that the number of differentially expressed genes (DEGs) induced by HIB radiation was less than that of XR group, but HIB radiation induced more differentially expressed proteins (DEPs). Both HIB and XR radiation activated genes of RNA silencing, double-strand break repair and cell catabolic process. DNA replication and cell cycle related genes were down-regulated. The genes of cell wall and external encapsulating structure were upregulated after HIB radiation. The gene expression of protein folding and glucan biosynthesis increased after XR radiation. Protein enrichment analysis indicated that HIB radiation resulted in differential protein enriched in photosynthesis and secondary metabolite biosynthesis pathways, while XR radiation induced differential protein of glyoxylate and dicarboxylate metabolism and carbon metabolism. After HIB and XR radiation, the genes of antioxidant system and terpenoid and polyketide metabolic pathways presented different expression patterns. HIB radiation led to the enrichment of non-homologous end-joining pathway. The results will contribute to understanding the biological effects of plants under space radiation.

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