MAEL gene contributes to bovine testicular development through the m5C-mediated splicing

文献类型： 外文期刊

第一作者： Lin, Feng

作者： Lin, Feng;Li, Ming;Gao, Tengyun;Fu, Tong;Deng, Tingxian;Li, Yali;Yang, Liu;Rushdi, Hossam E.;Riaz, Hasan;Yang, Liguo

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期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）

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年卷期： 2023 年 26 卷 2 期

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收录情况： SCI

摘要： Knowledge of RNA molecules regulating testicular development and spermatogenesis in bulls is essential for elite bull selection and an ideal breeding program. Herein, we performed direct RNA sequencing (DRS) to explore the functional characterization of RNA molecules produced in the testicles of 9 healthy Simmental bulls at three testicular development stages (prepuberty, puberty, and postpuberty). We identified 5,043 differentially expressed genes associated with testicular weight. These genes exhibited more alternative splicing at sexual maturity, particularly alternative 3' (A3) and 5' (A5) splice sites usage and exon skipping (SE). The expression of hub genes in testicular developmental stages was also affected by both m6A and m5C RNA modifications. We found m5C-mediated splicing events significantly (p < 0.05) increased MAEL gene expression at the isoform level, likely promoting spermatogenesis. Our findings highlight the complexity of RNA processing and expression as well as the regulation of transcripts involved in testicular development and spermatogenesis.

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