Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum

文献类型： 外文期刊

第一作者： Pan, Hongyu

作者： Pan, Hongyu;Wei, Yi;Xin, Furong;Zhou, Mingguo;Zhang, Shihong

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关键词： fusion chitinase;characterization and biocontrol ability;Trichothecium roseum;TRICHODERMA-HARZIANUM;FUNGAL PATHOGENS;ENCODING GENE;ENDOCHITINASE;EXPRESSION;CLONING

期刊名称：ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES （ 影响因子：1.649； 五年影响因子：1.66 ）

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收录情况： SCI

摘要： The antifungal mechanism of mycoparasitic fungi involves fungal cell wall degrading enzymes such as chitinases. Trichothecium roseum is an important mycoparasitic fungus with significant antifungal ability, but studies on chitinases of T roseum were poor. Here, we report a novel chitinase cDNA isolated from T roseum by PCR amplification based on conserved chitinase sequences. Southern blot analysis suggested that a single copy of the gene exists in the genome of T roseum. The deduced open reading frame of 1,143 nucleotides encodes a protein of 380 amino acids with a calculated molecular weight of 41.6 kDa. The fusion chitinase expressed in Escherichia coli has been purified by single-step chromatography. It has a pI of pH 5.4 and expresses a thermal stability, but is insensitive to pH in a broad pH range. According to expectation, E. coli efficiently yielded a high amount of active chitinase. Remarkably, the fusion chitinase offered high antifungal activity.

分类号： Q

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