Single base-resolution methylome of the silkworm reveals a sparse epigenomic map

文献类型： 外文期刊

第一作者： Xiang, Hui

作者： Xiang, Hui;Chen, Quan;Zhang, Guojie;Ye, Mingzhi;Guo, Guangwu;Li, Yingrui;Li, Ruiqiang;Zhang, Xiuqing;Ma, Lijia;Wang, Jun;Xiang, Hui;Li, Xin;Dong, Yang;Zhao, Li;Wang, Wen;Zhu, Jingde;Zhang, Hongyu;Yu, Jian;Sun, Jinfeng;Zhou, Xiaoyu;Ma, Kelong;He, Yinghua;Zhao, Yangxing;Guo, Shicheng;Zhu, Jingde;Dai, Fangyin;Li, Dong;Lin, Ying;Cheng, Daojun;Xia, Qingyou;Li, Muwang;Kristiansen, Karsten;Wang, Jun;Guo, Qiuhong;Jiang, Jianhao;Beck, Stephan

作者机构：

关键词： epigenetic regulation;sparse epigenomic map;single base-resolution methylome

期刊名称：NATURE BIOTECHNOLOGY （ 影响因子：54.908； 五年影响因子：50.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis(1) and human(2), demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.

分类号： Q1

  • 相关文献

[1]The miR-302/367 cluster: a comprehensive update on its evolution and functions. Gao, Zeqian,Zhu, Xueliang,Dou, Yongxi. 2015

[2]Beyond the genetic code in leaf senescence. Yolcu, Seher,Kim, Yun Ju,Li, Xiaojie,Li, Shengben. 2018

[3]Epigenetic regulation by the menin pathway. Feng, Zijie,Ma, Jian,Hua, Xianxin,Ma, Jian. 2017

[4]Epigenetic regulation of adipocyte differentiation and adipogenesis. Li, Hong-xing,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Wang, Cheng. 2010

[5]Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress. Fang, Yujie,Li, Jian,Jiang, Jinjin,Geng, Yulu,Wang, Jinglei,Wang, Youping,Fang, Yujie.

[6]CURVED CHIMERIC PALEA 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. Yan, Dawei,Zhang, Lin,Zeng, Longjun,Liu, Jiyun,Li, Qun,He, Zuhua,Zhang, Xiaoming,Ye, Shenghai,He, Zuhua.

作者其他论文 更多>>

微信小程序