Isolation, Culture and Biological Characteristics of Primordial Germ Cells from Beijing Fatty Chicken

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Guan, Weijun;Chen, Lina;Li, Xiangchen;Ma, Yue Hui;Wang, Ying;Hou, Lingling;Li, Chunying;Yue, Wenbin

作者机构：

关键词： alkaline phosphatase: 9001-78-9;EC 3.1.3.1;SSEA-4;TRA-1-81;yellow fluorescent protein;red fluorescent protein;periodic acid Schiff;SSEA-1: 98603-84-0;TRA-1-60;Dazl;Cvh;CDH;enhanced green protein;karyotype

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to explore the isolation and culture process of Beijing fatty chicken primordial germ cells (PGCs) and investigate their biological characteristics. The PGCs isolated from the genital ridges of Beijing fatty chicken (Gallus domesticus) embryos after 5.5 days of incubation were co-cultured with mice embryonic fibroblasts (MEF). The results showed that the PGCs of the Beijing fatty chicken were positive for periodic acid Schiff (PAS) and alkaline phosphatase (AKP) staining. These cells could proliferate for a prolonged time in vitro and maintain diploid karyotype. Immunocytochemical staining showed that they expressed SSEA-1, SSEA-4, TRA-1-60 and TRA-1-81, and real-time PCR showed that they expressed Cvh, CDH and Dazl. They could form simple embryoid bodies and differentiate into osteoblasts in vitro. In addition, after transfected with pEGFP-N3, pEYFP-N1 and pDsRed-N1 vectors by liposomal transfection, enhanced green, yellow and red fluorescent protein-positive cells could be visualized using a laser confocal microscope. The above results suggested that PGCs from the Beijing fatty chicken not only had strong self-renewal ability, but also had the potential to differentiate towards mesoblast cells. These cells are suitable for genetic manipulation as nuclear donors.

分类号： S814

  • 相关文献

[1]Threshold of Soil Olsen-P in Greenhouses for Tomatoes and Cucumbers. Wu, Xue-Ping,Wu, Hui-Jun,Wang, Xiao-Bin,Li, Yin-Kun,Zhang, Yan-Cai,Li, Ruo-Nan,Wang, Li-Ying,Zhai, Cai-Xia,Chen, Li-Li,Wu, Xue-Ping,Wu, Hui-Jun,Wang, Xiao-Bin.

[2]Differences of hyphal and soil phosphatase activities in drought-stressed mycorrhizal trifoliate orange (Poncirus trifoliata) seedlings. Wu, Qiang-Sheng,Zou, Ying-Ning,Wu, Qiang-Sheng,He, Xin-Hua,He, Xin-Hua,He, Xin-Hua,He, Xin-Hua.

[3]Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii). Feng, G.,Zhuang, P.,Zhang, L.,Duan, M.,Liu, J.,Huang, X.,Kynard, B.,Shi, X..

[4]Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish. Ye, Huan,Li, Chuang-Ju,Yue, Hua-Mei,Yang, Xiao-Ge,Wei, Qi-Wei,Ye, Huan,Yang, Xiao-Ge,Wei, Qi-Wei,Li, Chuang-Ju,Yue, Hua-Mei,Wei, Qi-Wei.

[5]Germ cell sex prior to meiosis in the rainbow trout. Li, Mingyou,Shen, Qian,Wong, Foong Mei,Xu, Hongyan,Hong, Ni,Hong, Yunhan,Zeng, Lingbing,Liu, Lin,Wei, Qiwei,Hong, Yunhan. 2011

[6]Chromosome painting of telomeric repeats reveals new evidence for genome evolution in peanut. Du Pei,Qi Zeng-jun,Du Pei,Li Li-na,Zhang Zhong-xin,Liu Hua,Qin Li,Huang Bing-yan,Dong Wen-zhao,Tang Feng-shou,Zhang Xin-you,Li Li-na. 2016

[7]Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution. He, Li,Zhao, Hainan,Zhang, Tao,Jiang, Jiming,Braz, Guilherme T.,Torres, Giovana A.,He, Li,Zhang, Tao,Semrau, Kassandra,Rouillard, Jean-Marie,Rouillard, Jean-Marie,Jiang, Jiming,Jiang, Jiming. 2018

[8]Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Sha Zhenxia,Ren Guocheng,Wang Xianli,Wang Na,Chen Songlin,Ren Guocheng,Wang Xianli. 2010

[9]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[10]Chromosome Variation from Sect. Chinenses (Rosa L.) through Chinese Old Garden Roses to Modern Rose Cultivars. Jian, H. Y.,Min, T.,Ting, Z.,Li, S. B.,Zhang, H.,Tang, K. X.. 2013

[11]Distribution of the tandem repeat sequences and karyotyping in cucumber (Cucumis sativus L.) by fluorescence in situ hybridization. Han, Y. H.,Liu, J. H.,Lu, J. Y.,Jin, W. W.,Han, Y. H.,Zhang, Z. H.,Huang, S. W..

[12]Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid. Zhu, Hua Ping,Lu, Mai Xin,Gao, Feng Ying,Huang, Zhang Han,Yang, Li Ping,Gui, Jian Fang.

[13]C-banding pattern and nucleolar organizer regions of Cynoglossus semilaevis Gunther, 1873. Wu Di,Zhang Shicui,Zhuang Zhimeng,Pang Qiuxiang,Wang Changliu,Wan Ruijing.

[14]Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis. Zhang, Bo,Wang, Xianli,Sha, Zhenxia,Yang, Changgeng,Liu, Shanshan,Wang, Na,Chen, Song-Lin,Zhang, Bo,Liu, Shanshan.

[15]Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus. Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,Peng, L. M.,Chen, S. L..

[16]Decaploidy in Rosa praelucens Byhouwer (Rosaceae) Endemic to Zhongdian Plateau, Yunnan, China. Jian, Hongying,Zhang, Hao,Tang, Kaixue,Li, Shufa,Wang, Qigang,Zhang, Ting,Qiu, Xianqin,Yan, Huijun.

[17]Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis). Wang, X. L.,Wang, N.,Sha, Z. X.,Chen, S. L.,Wang, X. L.. 2010

[18]Isolation and characterization of duck embryonic neural stem and progenitor cells. Hou, L.,Jin, D.,Gu, E.,Pu, Y.,Li, F.,Guan, W.,Ma, Y.,Hou, L.,Jin, D..

[19]Karyotype Analysis of Mediterranean Buffalo and Its Hybrids. Huang, Fenxiang,Yang, Bingzhuang,Tan, ZhengZhun,Huang, Jian,Li, Hui,Pang, Chunying,Shang, Jianghua,Liang, Xianwei. 2013

[20]Karyotype and Flow Cytometry Analysis of the Natural Populations of Rosa beggeriana and Rosa laxa in Xinjiang, China. Yang, S.,Han, Y. J.,Yang, S. H.,Ge, H.. 2014

作者其他论文 更多>>

微信小程序