Study on the methods for the determination of corn root-washing's mugineic acid

文献类型： 外文期刊

第一作者： Wang Wei-Ting

作者： Wang Wei-Ting;Zhang Chun-Qing;Li Peng

作者机构：

关键词： corn; mugineic acids; spectrophotometry; inductively coupled plasma-atomic emission spectrometry; ultraviolet detection-high performance liquid chromatography

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2008 年 36 卷 1 期

页码：

收录情况： SCI

摘要： The accurate and quick measurement of mugineic acids (MAs) of graminaceous plants was very important for the breeding of -high-efficient usage of iron nutrition and physiological research of Fe-deficient tolerance. According to the chelate characteristic of Fe ( M) with mugineic acid, a new indirect method of inductively coupled plasma-atomic emission spectrometry (ICP-AES) and a modified direct method of UV detection-high performance liquid chromatography(UV-HPLC) for the measurement of MAs or deoxymugineic acid (DMA) were established in corn inbred lines. The comparison of these two methods with traditional spectrophotometry was also made. The results showed that the ICP-AES method, using Fe, 0, as the dissoluble object instead of the traditional Fe (OH)(3), was more stable t (ICP-AES) = 4.080 > t(0.05) (15) = 2.131 and repeatalble (RSD (n = 3) = 3.3). It was suggested that ICP-AES was a simple and quick method for evaluation of the MAs content in corn and other graminaceous crops. The modified UV-HPLC was also precise and efficient, and could be used for the direct mesurement of graminaceous's deoxymugineic acid.

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