Nanopore detects.-radiation inhibited HIV-1 protease activity

文献类型： 外文期刊

第一作者： Li, Minghan

作者： Li, Minghan;Li, Minghan;Li, Wei;Xiao, Yicen;Liu, Qianshan;Liang, Liyuan;Wang, Deqiang;Wang, Liang;Li, Minghan;Li, Wei;Xiao, Yicen;Liu, Qianshan;Liang, Liyuan;Wang, Deqiang;Wang, Liang;Huang, Wenli;Wang, Liang

作者机构：

关键词： HIV-1 protease; GagPol precursor; Enzyme inactivation; Co-60 gamma-radiation; Nanopore

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.545； 五年影响因子：10.722 ）

ISSN： 0956-5663

年卷期： 2021 年 194 卷

页码：

收录情况： SCI

摘要： Inhibition of HIV-1 protease (PR) activity is realized by exposure to Co-60 gamma-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows similar to 50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of gamma-radiation (0.1K Gy), and the values reach to over 90% with high gamma-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of gamma-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.

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