Mucosal immune responses induced by oral administration of recombinant Lactococcus lactis expressing the S1 protein of PDCoV

文献类型： 外文期刊

第一作者： Zhai, Kaige

作者： Zhai, Kaige;Zhang, Zhongwang;Liu, Xinsheng;Lv, Jianliang;Zhang, Liping;Li, Jiahao;Ma, Zhongyuan;Wang, Yonglu;Guo, Huichen;Zhang, Yongguang;Pan, Li

作者机构：

关键词： PDCoV; S1 protein; Lactococcus lactis; Oral mucosal vaccine

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 578 卷

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus is an evolving coronavirus that primarily infects the intestine and may lead to intestinal disease in piglets. Up to now, no commercial vaccination is readily accessible to protect against the spread of PDCoV. Lactococcus lactis has been shown to have good immune efficacy and safety and can be used as a genetically engineered vaccine to deliver antigens. In this research, we utilized L. lactis NZ9000 to provide the S1 protein orally and improved the delivery efficiency by connecting the M cell targeting ligand Co1 with the S1 protein of PDCoV in tandem to obtain the recombinant protein S1-Co1. We successfully constructed two recombinant strains capable of expressing PDCoV-S1 and PDCoV-S1-Co1 proteins (i.e., L. lactis NZ9000-S1 and L. lactis NZ9000-S1-Co1), and their immunogenic capacity was evaluated in mice. Our study shows that Lactococcus is an advantageous bacterial live vector vaccine and is anticipated as a potential PDCoV vaccination option.

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