An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome

文献类型： 外文期刊

第一作者： Lv, Jian

作者： Lv, Jian;Zhan, Jianwu;Sun, Zhi;Yuan, Shishan;Lv, Jian;Liu, Weiquan

作者机构：

关键词： EQUINE-ARTERITIS-VIRUS;UNITED-STATES;VACCINE;IDENTIFICATION;STRAIN;CHINA;HETEROGENEITY;CONSTRUCTION;SEQUENCE;REGIONS

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Since May 2006, a so-called 'porcine high fever syndrome' (PHFS) has spread all over China. The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) was believed to be the main causative agent, although the involvement of other pathogens was not formally excluded. The genome of a representative Chinese PRRSV strain, named JX143, was sequenced and used to develop infectious cDNA clones, pJX143 and pJX143M, with the latter containing an engineered Mlul site that served as a genetic marker. In various virological assays, the rescued viruses, vJX143 and vJX143M, were indistinguishable from their parental virus. Animal experiments showed that these recombinant viruses retained the high pathogenicity and induced the typical clinical symptoms observed during PHFS outbreaks. This is the first report describing infectious cDNA clones of this highly pathogenic PRRSV. Our results unambiguously fulfil Koch's postulates and define highly pathogenic PRRSV as the aetiological agent of PHFS in China.

分类号： R37

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