Laoxianghuang polysaccharide promotes the anti-inflammatory cytokine interleukin-10 in colitis via gut microbial linoleic acid

文献类型： 外文期刊

第一作者： Zhang, Yuwei

作者： Zhang, Yuwei;Fan, Bei;Wang, Fengzhong;Zheng, Yang;Zhang, Yuwei;Yang, Yiren;Song, Jiangping;Yu, Wenqing;Li, Yaqian;Liu, Denghong;Gao, Jie

作者机构：

关键词： Laoxianghuang polysaccharide; DSS-induced colitis; Gut microbiota; Linoleic acid; IL-10

期刊名称：PHYTOMEDICINE （ 影响因子：8.3； 五年影响因子：8.0 ）

ISSN： 0944-7113

年卷期： 2024 年 135 卷

页码：

收录情况： SCI

摘要： Background: Our previous study found that the polysaccharide from Laoxianghuang (LP), fermented fruit of bergamot (traditional Chinese medicine and food), can alter gut microbiota and regulate short-chain fatty acids (SCFAs) in vitro. Nevertheless, there is a paucity of reports on the impact of LP on gut microbiota in vivo. Purpose: To analyze the structures of LP, investigate the influence of LP on the damaged intestinal barrier in DSSinduced colitis mice, and further explore its potential mechanisms. Methods: We analyzed the physicochemical properties of purified LP by HPLC, SEM, and FT-IR spectrum. Then, to assess the effect of LP in DSS-induced colitis mice, we observed the damage to the colon tissue, measured inflammatory cytokines and tight junction protein expression through RT-qPCR as well as immunofluorescent staining, and investigated the influence of LP on altering gut microbiota and metabolites using 16 s rRNA sequencing and HPLC-MS/MS. Ultimately, the impact of linoleic acid on inflammatory cytokines was confirmed by the LPS-induced RAW264.7 cells. Results: LP, mainly galactoglucan, could inhibit weight loss and colon shortening, decrease levels of tumor necrosis factor-alpha (TNF-alpha), increase levels of interleukin-10 (IL-10) and the intestinal acetic acid and butyric acid, and promote the expression of tight junction proteins ZO-1 and Claudin-1. Meanwhile, LP enhanced the abundance of beneficial bacteria including Romboutsia, Eubacterium_coprostanoligenes_group, and Akkermansia, and regulated linoleic acid metabolism to increase the linoleic acid level. In vitro cell experiment proved that linoleic acid could elevate the level of IL-10 and inhibit inflammatory responses. Conclusions: Our results suggested that LP effectively alleviated colitis by promoting the anti-inflammatory cytokine interleukin-10 via gut microbiota-mediated linoleic acid metabolism.

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