Selection and Genetic Analysis of High Polysaccharide-Producing Mutants in Inonotus obliquus

文献类型： 外文期刊

第一作者： Hua, Lanlan

作者： Hua, Lanlan;Shi, Hongling;Lin, Qing;Wang, Haozhong;Gao, Yan;Zeng, Jun;Lou, Kai;Huo, Xiangdong;Hua, Lanlan;Shi, Hongling;Lin, Qing;Gao, Yan;Zeng, Jun;Lou, Kai;Huo, Xiangdong;Wang, Haozhong

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关键词： ARTP; genome resequencing; polysaccharide

期刊名称：MICROORGANISMS （ 影响因子：4.1； 五年影响因子：4.5 ）

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年卷期： 2024 年 12 卷 7 期

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收录情况： SCI

摘要： Inonotus obliquus, a medicinal fungus, has garnered significant attention in scientific research and medical applications. In this study, protoplasts of the I. obliquus HS819 strain were prepared using an enzymatic method and achieved a regeneration rate of 5.83%. To enhance polysaccharide production of I. obliquus HS819, atmospheric and room temperature plasma (ARTP) technology was employed for mutagenesis of the protoplasts. Through liquid fermentation, 32 mutant strains exhibiting diverse characteristics in morphology, color of the fermentation broth, mycelial pellet size, and biomass were screened. Secondary screening identified mutant strain A27, which showed a significant increase in polysaccharide production up to 1.67 g/L and a mycelial dry weight of 17.6 g/L, representing 137.67% and 15% increases compared to the HS819 strain, respectively. Furthermore, the fermentation period was reduced by 2 days, and subsequent subculture cultivation demonstrated stable polysaccharide production and mycelial dry weight. The genome resequencing analysis of the HS819 strain and mutant strain A27 revealed 3790 InDel sites and mutations affecting 612 functional genes associated with polysaccharide synthesis. We predict that our findings will be helpful for high polysaccharide production through genetic engineering of I. obliquus.

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