Transcriptome alterations in zebrafish gill after exposure to different sizes of microplastics

文献类型： 外文期刊

第一作者： Xue, Ying-Hao

作者： Xue, Ying-Hao;Xue, Ying-Hao;Jia, Tao;Xu, Zhi-Yu;Yang, Ning;Sun, Zhan-Xiang;Feng, Liang-Shan;Wen, Xin-Li

作者机构：

关键词： microplastic; Danio rerio; KEGG; oxidative stress

期刊名称：JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING （ 影响因子：2.582； 五年影响因子：2.518 ）

ISSN： 1093-4529

年卷期： 2022 年 57 卷 5 期

页码：

收录情况： SCI

摘要： Most studies on microplastics (MPs) focused on gut, liver, and brain, and MPs toxicity was size-dependent, but less has been reported on gill. Here, zebrafish were exposed to three sizes of MPs (45-53 mu m, 90-106 mu m, and 250-300 mu m). Next, comparative transcriptome analysis and determination of physiological indices were performed in zebrafish gills to elucidate the size-associated toxicity of MPs to fish gills. Compared with the control, 60, 344, and 802 differentially expressed genes (DEGs) were identified after exposure to 45-53 mu m, 90-106 mu m, and 250-300 mu m MPs for 5 days, respectively. More DEGs in treatment with bigger MPs suggested that bigger MPs might induce more changes in zebrafish gills than smaller ones. These DEGs were significantly enriched in the FoxO signaling, cellular senescence, circadian rhythm and p53 signaling pathways. Besides, 90-106 mu m and 250-300 mu m MPs treatments inhibited the cell cycle and prevented the apoptosis. The GSH content significantly increased after MPs exposure, suggesting the induction of oxidative stress. AChE and Na+/K+-ATPase activities were significantly lowered in all MPs treatments than in the control, suggesting the inhibition of neurotransmission and ion regulation. These changes might negatively influence the normal functioning of gills, such as osmoregulation, ion regulation, and respiration.

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