Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation
文献类型： 外文期刊
第一作者： Wang, Junzhi
作者： Wang, Junzhi;Qi, Jiale;Fang, Yuhang;Bai, Lianyang;Pan, Lang;Wang, Junzhi;Bai, Lianyang;Bai, Lianyang;Nyporko, Alex;Yu, Qin
作者机构：
关键词： cytochrome P450s; mesosulfuron-methyl; enhanced herbicide metabolism; transcription factor
期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）
ISSN： 0960-7412
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Frequent herbicide use selects for herbicide resistance in weeds. Cytochrome P450s are important detoxification enzymes responsible for herbicide resistance in plants. We identified and characterized a candidate P450 gene (BsCYP81Q32) from the problematic weed Beckmannia syzigachne to test whether it conferred metabolic resistance to the acetolactate synthase-inhibiting herbicides mesosulfuron-methyl, bispyribac-sodium, and pyriminobac-methyl. Transgenic rice overexpressing BsCYP81Q32 was resistant to the three herbicides. Equally, rice overexpressing the rice ortholog gene OsCYP81Q32 was more resistant to mesosulfuron-methyl. Conversely, an OsCYP81Q32 gene knockout generated using CRISPR/Cas9 enhanced mesosulfuron-methyl sensitivity in rice. Overexpression of the BsCYP81Q32 gene resulted in enhanced mesosulfuron-methyl metabolism in transgenic rice seedlings via O-demethylation. The major metabolite, demethylated mesosulfuron-methyl, was chemically synthesized and displayed reduced herbicidal effect in plants. Moreover, a transcription factor (BsTGAL6) was identified and shown to bind a key region in the BsCYP81Q32 promoter for gene activation. Inhibition of BsTGAL6 expression by salicylic acid treatment in B. syzigachne plants reduced BsCYP81Q32 expression and consequently changed the whole plant response to mesosulfuron-methyl. Sequence polymorphisms in an important region of the BsTGAL6 promoter may explain the higher expression of BsTGAL6 in resistant versus susceptible B. syzigachne plants. Collectively, the present study reveals the evolution of an herbicide-metabolizing and resistance-endowing P450 and its transcription regulation in an economically important weedy plant species.
分类号：
- 相关文献
作者其他论文 更多>>
-
Revealing the synergistic effect between radical and non-radical species of sulfur-doped carbon nitride for ciprofloxacin removal: Based on density functional theory study
作者：Zeng, Hao;Zhou, Zhanpeng;Li, Wenbo;Li, Ling;Tang, Rongdi;Xiong, Sheng;Gong, Daoxin;Deng, Yaocheng;Zhou, Zhanpeng;Li, Wenbo;Li, Ling;Xiong, Sheng;Huang, Ying;Tang, Rongdi;Tang, Rongdi;Zeng, Hao;Bai, Lianyang
关键词：Photocatalytic; Ciprofloxacin; Cooperative degradation; Singlet oxygen; Density functional theory
-
Bioremediation of paddy soil with amphitropic mixture markedly attenuates rice cadmium: Effect of soil cadmium removal and Fe/S-cycling bacteria in rhizosphere
作者：Zhang, Feng;Zhang, Feng;Deng, Yan;Jiang, Huidan;Peng, Rui;Bai, Lianyang
关键词：Cd; Paddy soil; Rice; Microbial mixture; Bioremediation
-
Bromine Atoms Decorated Pyene-based Covalent Organic Frameworks for Accelerated Photocatalytic H2 Production
作者：Deng, Xile;Bai, Lianyang;Gao, Nan;Gao, Nan
关键词：Br Atoms decorated; covalent organic frameworks; Exciton binding energy; Photocatalytic H-2 Production
-
Impact of chronic exposure to field level glyphosate on the food consumption, survival, gene expression, gut microbiota, and metabolomic profiles of honeybees
作者：Ma, Changsheng;Bai, Lianyang;Ma, Changsheng;Li, Zuren;Bai, Lianyang;Gu, Gaoying;Gu, Gaoying;Chen, Sihao;Chen, Sihao;Shi, Xiaoyu;Li- Byarlay, Hongmei
关键词：Herbicide; Pollinator; Behavior; Potential mechanism
-
A first-in-class dimethyl 2-acetamido terephthalate inhibitor targeting Conyza canadensis SHMT1 with a novel herbicidal mode-of-action
作者：Luo, Dingfeng;Bai, Zhendong;Bai, Haodong;Liu, Na;Han, Jincai;Ma, Changsheng;Wu, Di;Bai, Lianyang;Li, Zuren;Luo, Dingfeng;Liu, Na;Han, Jincai;Ma, Changsheng;Bai, Lianyang;Li, Zuren
关键词：Herbicide target; Serine hydroxymethyltransferase (SHMT); Crystal structure; In silico screening; Synthesis
-
Molecular Mechanism of Pyrroloquinoline Quinone-Mediated Rice Tolerance to Imidazolinone Herbicide Imazamox
作者：Li, Sifu;Tang, Tao;Ma, Guolan;Liu, Ducai;Peng, Yajun;Li, Sifu;Liu, Yang;Zhang, Yuzhu;Bai, Lianyang;Li, Sifu;Hu, Shiyuan;Luo, Kai;Liu, Yang;Zhang, Yuzhu;Bai, Lianyang;Hu, Shiyuan;Luo, Kai
关键词：imidazolinone herbicide; phytotoxicity; pyrroloquinoline quinone; RNA-seq; RT-qPCR; enzymatic activity
-
A reference genome of Commelinales provides insights into the commelinids evolution and global spread of water hyacinth (Pontederia crassipes)
作者：Huang, Yujie;Xie, Lingjuan;Shang, Nianmin;Wu, Dongya;Ye, Chuyu;Fan, Longjiang;Huang, Yujie;Xie, Lingjuan;Shang, Nianmin;Wu, Dongya;Ye, Chuyu;Fan, Longjiang;Huang, Yujie;Fan, Longjiang;Guo, Longbiao;Rudell, Eduardo Carlos;Junior, Aldo Merotto;Okada, Kazunori;Zhu, Qian-Hao;Song, Beng-Kah;Cai, Daguang;Bai, Lianyang
关键词：Pontederia crassipes or Eichhornia crassipes; Commelinales; reference genome; phylogeny of the commelinids; genetic diversity; karyotypes
