Development and Application of Prime Editing in Plants

文献类型： 外文期刊

第一作者： Liu, Tingting

作者： Liu, Tingting;Zou, Jinpeng;Yang, Xi;Wang, Kejian;Wang, Chun;Liu, Tingting;Yang, Xi;Rao, Yuchun

作者机构：

关键词： prime editing; CRISPR/Cas; precision genome editing; crop breeding

期刊名称：RICE SCIENCE （ 影响因子：4.8； 五年影响因子：5.5 ）

ISSN： 1672-6308

年卷期： 2023 年 30 卷 6 期

页码：

收录情况： SCI

摘要： Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing has greatly accelerated progress in plant genetic research and agricultural breeding by enabling targeted genomic modifications. Moreover, the prime editing system, derived from the CRISPR/Cas system, has opened the door for even more precise genome editing. Prime editing has the capability to facilitate all 12 types of base-to-base conversions, as well as desired insertions or deletions of fragments, without inducing double-strand breaks and requiring donor DNA templet. In a short time, prime editing has been rapidly verified as functional in various plants, and can be used in plant genome functional analysis as well as precision breeding of crops. In this review, we summarize the emergence and development of prime editing, highlight recent advances in improving its efficiency in plants, introduce the current applications of prime editing in plants, and look forward to future prospects for utilizing prime editing in genetic improvement and precision molecular breeding.

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