Encapsulin nanoparticle-conjugated p54 protein boosts immune responses against African swine fever virus

文献类型： 外文期刊

第一作者： Zhang, Yue

作者： Zhang, Yue;Ru, Yi;Zhao, Longhe;Hao, Rongzeng;Yang, Yang;Shen, Chaochao;Shi, Zhengwang;Zheng, Haixue;Zheng, Haixue;Shen, Chaochao;Shi, Zhengwang;Zheng, Haixue

作者机构：

关键词： African swine fever virus; p54 protein; Ferritin; Targeted lymph nodes; Encapsulin nanoparticle-based antigens; Immune activation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 311 卷

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) poses a severe threat to global swine production, with no commercially approved vaccine. The ASFV p54 protein is critical for viral entry and trafficking, interacting with the dynein light chain 8 (DLC8) to facilitate replication and spread within host cells. Despite advances in vaccine development, current strategies struggle to induce sustained immune responses. In this study, we utilized SpyTag/ SpyCatcher technology to covalently conjugate the ASFV p54 protein to ferritin and encapsulin nanoparticles (F/ E-p54), aiming to enhance immune recognition and optimize antigen presentation. The conjugated nanoparticles exhibited a 60-fold enhancement in receptor binding affinity over the soluble p54 monomer, improving targeted delivery to lymphoid tissues and stimulating T follicular helper (Tfh) cells and germinal center B (GCB) cells. The encapsulin-based nanoparticle approach elicited strong B- and T-cell responses, promoting prolonged immune activation and the production of neutralizing antibodies. Additionally, antibodies generated by the F/E-p54 conjugates effectively inhibited both genotype II and recombinant I/II ASFV strains. These findings highlight the potential of nanoparticle-based vaccines, particularly encapsulin-conjugated systems, as promising platforms for advancing ASFV vaccine development and guiding future immunization strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序