Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency

文献类型： 外文期刊

第一作者： Nie, Zhi

作者： Nie, Zhi;Luo, Bowen;Zhang, Xiao;Wu, Ling;Liu, Dan;Guo, Jialei;He, Xuan;Gao, Duojiang;Gao, Shiqiang;Gao, Shibin;Nie, Zhi

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关键词： maize; phosphate deficiency; transcriptome; proteome

期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 影响因子：2.081； 五年影响因子：2.561 ）

ISSN： 1467-3037

年卷期： 2021 年 43 卷 2 期

页码：

收录情况： SCI

摘要： A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.

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