PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice
文献类型： 外文期刊
第一作者： Cheng, Kaihui
作者： Cheng, Kaihui;Yu, Zhijun;Sun, Weiyang;Xin, Yue;Huang, Jing;Zhang, Kun;Li, Xue;Yang, Songtao;Wang, Tiecheng;Zheng, Xuexing;Wang, Hualei;Qian, Jun;Gao, Yuwei;Xia, Xianzhu;Cheng, Kaihui;Yu, Zhijun;Qin, Chuan;Xia, Xianzhu;Yu, Zhijun;Qin, Chuan;Xia, Xianzhu;Zhang, Qianyi;Chen, Hualan;Chai, Hongliang;Hua, Yuping
作者机构：
关键词： Avian influenza virus; H6N1; Mice; Pathogenicity
期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）
ISSN： 0042-6822
年卷期： 2014 年 468 卷
页码：
收录情况： SCI
摘要： H6N1 avian influenza viruses (AIVs) may pose a potential human risk as suggested by the first documented naturally-acquired human H6N1 virus infection in 2013. Here, we set out to elucidate viral determinants critical to the pathogenesis of this virus using a mouse model. We found that the recombinant H6N1 viruses possessing both the PA-T97I and PB2-E627K substitutions displayed the greatest enhancement of replication in vitro and in vivo. Polymerase complexes possessing either PB2-E627K, PA-T97I, and PB2-E627K/PA-T97I displayed higher virus polymerase activity when compared to the wild-type virus, which may account for the increased replication kinetics and enhanced virulence of variant viruses. Our results demonstrate that PB2-E627K and PA-T97I enhance the ability of H6N1 virus to replicate and cause disease in mammals. Influenza surveillance efforts should include scrutiny of these regions of PB2 and PA because of their impact on the increased virulence of H6N1 AIVs in mice. (C) 2014 Elsevier Inc. All rights reserved.
分类号： R37
- 相关文献
作者其他论文 更多>>
-
A broad-spectrum vaccine candidate against H5 viruses bearing different sub-clade 2.3.4.4 HA genes
作者：Zhang, Yuancheng;Cui, Pengfei;Shi, Jianzhong;Zeng, Xianying;Jiang, Yongping;Chen, Yuan;Zhang, Jie;Wang, Congcong;Wang, Yan;Tian, Guobin;Chen, Hualan;Kong, Huihui;Deng, Guohua
关键词：
-
Quantitative Determination of Cd Using Energy Dispersion XRF Based on Gaussian Mixture Clustering-Multilevel Model Recalibration
作者：Gao, Yunbing;Zhao, Yanan;Pan, Yuchun;Sun, Wenbin;Zhao, Xiande;Liu, Xiaoyang;Li, Xue;Mao, Xuefei
关键词：
-
Rotary Tillage Plus Mechanical Transplanting Practices Increased Rice Yields with Lower CH4 Emission in a Single Cropping Rice System
作者：Shi, Linlin;Dong, Linlin;Shen, Yuan;Tao, Yueyue;Wang, Haihou;Shi, Linlin;Lu, Changying;Zhang, Jun;Huang, Jing
关键词：tillage; rice yield; methane; methanogens; methanotrophs
-
Review on liquid-core waveguide technology and its application for spectroscopic analysis
作者：Mu, Bingyu;Li, Xue;Liu, Jixin;Mao, Xuefei;Mu, Bingyu;Li, Xue;Liu, Jixin;Mao, Xuefei;Liu, Jixin
关键词：liquid core waveguide; total internal reflection; long path-length; teflon AF; Spectroscopic analysis
-
Genetics and Pathogenicity of Influenza A (H4N6) Virus Isolated from Wild Birds in Jiangsu Province, China, 2023
作者：Song, Xingdong;Chai, Hongliang;Li, Yanbing;Tian, Jingman;Li, Minghui;Bai, Xiaoli;Zhao, Zhiguo;Shi, Jianzhong;Zeng, Xianying;Tian, Guobin;Guan, Yuntao;Cui, Pengfei;Deng, Guohua;Liu, Liling;Li, Yanbing;Chen, Hualan
关键词：
-
Stimuli-responsive biodegradable silica nanoparticles: From native structure designs to biological applications
作者：Qi, Qianhui;Wang, Wei;Shen, Qian;Geng, Jiaying;An, Weizhen;Wu, Qiong;Yu, Changmin;Shen, Qian;Geng, Jiaying;An, Weizhen;Wu, Qiong;Yu, Changmin;Qi, Qianhui;Yu, Changmin;Wang, Nan;Zhang, Yu;Li, Xue;Li, Lin
关键词：Biodegradation; Silica nanoparticles; Stimuli -responsive; Multiple frameworks; Biological applications
-
Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1
作者：Liu, Wenxiao;Zhang, Kun;Cheng, Jing;Cheng, Chunjie;Jiang, Bo;Zhou, Linyi;Li, Yongqing;Liu, Wenxiao;Cheng, Jing;Jiang, Bo;Zhou, Linyi;Li, Yongqing;Zhang, Kun;Yu, Shiqiang
关键词：bovine herpesvirus 1; diagnosis; lateral flow strip; time-resolved fluorescent microspheres; monoclonal antibody
