Suppression of Bta11975, an alpha-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

文献类型： 外文期刊

第一作者： Lu, DingYiHui

作者： Lu, DingYiHui;Yue, Hao;Huang, LiPing;Zhang, DeYong;Shi, XiaoBin;Liu, Yong;Zhang, ZhanHong;Zhang, Youjun;Li, Fan;Yan, Fei;Zhou, XuGuo

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关键词： alpha-glucosidase; RNA interference; tomato chlorosis virus; Bemisia tabaci

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2021 年 77 卷 11 期

页码：

收录情况： SCI

摘要： BACKGROUND: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an alpha-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene. RESULTS: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci. CONCLUSIONS: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

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