Lactobacillus rhamnosus MN-431 Metabolic Tryptophan Alleviates Complementary Food-Induced Diarrhea through PXR-NF-kappa B Pathway and AHR-Th17 Cell Response Pathways

文献类型： 外文期刊

第一作者： Niu, Haiyue

作者： Niu, Haiyue;Zhou, Xiaohong;Liu, Ping;Wu, Yifan;Lyu, Linzheng;Zhang, Jiliang;Chen, Shiwei;Han, Xue;Liu, Tongjie;Zhang, Zhe;Gong, Pimin;Zhang, Lanwei

作者机构：

关键词： aryl hydrocarbon receptor; IAld; L; rhamnosus MN-431; PXR; skatole

期刊名称：MOLECULAR NUTRITION & FOOD RESEARCH （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN： 1613-4125

年卷期： 2023 年 67 卷 13 期

页码：

收录情况： SCI

摘要： ScopeLactobacillus rhamnosus MN-431 tryptophan broth culture (MN-431 TBC) can prevent complementary food-induced diarrhea (CFID). However, it is not clear whether this effect is related to indole derivatives. Methods and resultsIn this study, the anti-CFID effects of different components in MN-431 TBC including MN-431 cells, unfermented tryptophan broth, and supernatant of MN-431 TBC (MN-431 TBS) are investigated. Only MN-431 TBS can significantly prevent CFID, indicating that indole derivatives produced by MN-431 can exert antidiarrheal effects. Intestinal morphological analysis reveals that MN-431 TBS can increase the number of goblet cells, height of ileal villi, and length of rectal glands while also increasing the expression of ZO-1 in colon. Furthermore, HPLC analysis reveals the indole derivatives in MN-431 TBS are IAld and skatole. Cell experiments demonstrate that MN-431 TBS promotes the transcription of aryl hydrocarbon receptor (AHR) and pregnane X receptor (PXR), comparable to the synergistic effect of IAld and skatole. MN-431 TBS can activate AHR and reduces the concentrations of Th17 cell-inflammatory factors IL-17A and IL-21 in intestine and IL-17F, IL-21, and IL-22 in serum. MN-431 TBS can also activate PXR and reduces the concentrations of TNF-alpha and IL-6 in intestine and serum. ConclusionMN-431 TBS, containing IAld and skatole, can exert anti-CFID effects through the AHR-Th17 and PXR-NF-kappa B pathways.

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