miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

文献类型： 外文期刊

第一作者： Abdurahman, Anwar

作者： Abdurahman, Anwar;Abdurahman, Anwar;Abdurahman, Anwar;Aierken, Wusimanjiang;Zhang, Fei;Obulkasim, Rahmantay;Aniwashi, Jueken;Sulayman, Ablat

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关键词： BMPR1B; granulosa cell; miR-1306; apoptosis; sheep

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.772； 五年影响因子：4.933 ）

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年卷期： 2022 年 13 卷

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收录情况： SCI

摘要： Bone morphogenetic protein receptor type-1B (BMPR1B) is one of the major gene for sheep prolificacy. However, few studies investigated its regulatory region. In this study, we reported that miR-1306 is a direct inhibitor of BMPR1B gene in the ovine granulosa cells (ovine GCs). We detected a miRNA response element of miR-1306 in the 3' untranslated region of the ovine BMPR1B gene. Luciferase assay showed that the ovine BMPR1B gene is a direct target of miR-1306. qPCR and western blotting revealed that miR-1306 reduces the expression of BMPR1B mRNA and protein in the ovine granulosa cells. Furthermore, miR-1306 promoted cell apoptosis by suppressing BMPR1B expression in the ovine granulosa cells. Overall, our results suggest that miR-1306 is an epigenetic regulator of BMPR1B, and may serve as a potential target to improve the fecundity of sheep.

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