Molecular Dissection of the Pathogen-Inducible 3-Deoxyanthocyanidin Biosynthesis Pathway in Sorghum

文献类型： 外文期刊

第一作者： Liu, Hongjia

作者： Liu, Hongjia;Du, Yegang;Chu, Hung;Shih, Chun Hat;Wong, Yu Wai;Wang, Mingfu;Lo, Clive;Liu, Hongjia;Tao, Yuezhi;Chu, Ivan K.

作者机构：

关键词： salicylic acid: 69-72-7;NADPH: 53-57-6;methyl jasmonate: 1211-29-6;anthocyanin: synthesis;3-deoxyanthocyanidin: synthesis;accumulation;phytoalexin;Gene Name;Sorghum DFR gene [Gramineae]: Sorghum dihydroflavonol 4-reductase gene;Sorghum chalcone synthase gene [Gramineae]: expression;Sorghum SbDFR1 gene [Gramineae]: expression;Sorghum SbDFR3 gene [Gramineae]: expression;Sorghum flavanone3-hydroxylase;gene [Gramineae]: expression;GenBank sequence data;pathogen-induced chemical accumulation

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： 3-Deoxyanthocyanidins are the unique phytoalexins synthesized by sorghum in response to fungal inoculation. They are structurally related to anthocyanins but the final steps of their pathogen-inducible biosynthesis are not fully understood. We have identified new flavonoid structural genes from the recently completed sorghum BTx623 genome sequence. The biochemical functions of the different expressed sorghum genes were established in planta by complementation in the appropriate Arabidopsis transparent testa mutants. There is a family of nine chalcone synthase genes which are all inducible by fungal inoculation in sorghum seedlings. Specific dihydroflavonol 4-reductase (DFR) genes responsive to conditions which stimulated anthocyanin accumulation (SbDFR1) or 3-deoxyanthocyanidin production (SbDFR3) were identified. Recombinant SbDFR1 and SbDFR3 were found to function as typical DFRs by accepting dihydroflavonol substrates. On the other hand, both DFRs showed substantially lower but detectable NADPH-dependent activities toward flavanones. Reduction of flavanones to flavan-4-ols is a reaction step required for 3-deoxyanthocyanidin production. Flavanone 3-hydroxylase (F3H) converts flavanones to dihydroflavonols for anthocyanin biosynthesis. In sorghum seedlings, expression of two F3H genes was either absent or strongly suppressed during the accumulation of 3-deoxyanthocyanidins. Under such conditions, most flavanones are expected to be reduced by the pathogen-induced SbDFR3 for the formation of flavan-4-ols. Our work also revealed that 3-deoxyanthocyanidin accumulation and SbDFR3 expression were induced by methyl jasmonate treatment in sorghum roots but the stimulation effects were antagonized by salicylic acid.

分类号： Q945

  • 相关文献

[1]Comparative Transcriptomic Profiling of a Salt-Tolerant Wild Tomato Species and a Salt-Sensitive Tomato Cultivar. Sun, Wei,Zhu, Huishan,Liu, Aihua,Hua, Xuejun,Xu, Xinna,Liu, Lei,Li, Junming.

[2]The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis. Gao, Jian-Jie,Shen, Xue-Fang,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Yao, Quan-Hong,Gao, Jian-Jie,Shen, Xue-Fang,Zhang, Zhen,Xu, Jing,Zheng, Jian-Li,Yao, Quan-Hong,Yao, Quan-Hong.

[3]Phytochromes Regulate SA and JA Signaling Pathways in Rice and Are Required for Developmentally Controlled Resistance to Magnaporthe grisea. Xie, Xian-Zhi,Xue, Yan-Jiu,Zhou, Jin-Jun,Zhang, Bin,Xie, Xian-Zhi,Zhou, Jin-Jun,Zhang, Bin,Xue, Yan-Jiu,Chang, Hong,Takano, Makoto.

[4]Analysis of differentially expressed genes in Oryza meyeriana in response to infection by Xanthomonas oryzae pv. oryzae. Cheng, Z.,Tang, K.,Yan, H.,Fu, J.,Ying, F.,Huang, X.,Tang, K.,Huang, X..

[5]Preliminary study on phytoalexin induction in cucumber. Wang, JB,Wang, BC,Zhao, HC,Li, BJ.

[6]Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk. Li, Yangrui,Yang, Litao.

[7]Gene expression pattern in response to wounding, methyl jasmonate and ethylene in the bark of Hevea brasiliensis. Duan, Cuifang,Rio, Maryannick,Leclercq, Julie,Oliver, Gerald,Montoro, Pascal,Duan, Cuifang,Bonnot, Francois.

[8]Forced expression of Mdmyb10, a myb transcription factor gene from apple, enhances tolerance to osmotic stress in transgenic Arabidopsis. Gao, Jian-Jie,Peng, Ri-He,Xiong, Ai-Sheng,Zhu, Bo,Yao, Quan-Hong,Gao, Jian-Jie,Zhang, Zhen,Xu, Jing,Yao, Quan-Hong.

[9]Salicylic acid induces stomatal closure by modulating endogenous hormone levels in cucumber cotyledons. Hao, J. H.,Wang, X. L.,Dong, C. J.,Zhang, Z. G.,Shang, Q. M..

[10]Isolation and characterization of GoDREB encoding an ERF-type protein in forage legume Galegae orientalis. Wang, Xuemin,Chen, Xiaofang,Wang, Zan,Gao, Hongwen,Nikolay, Dzyubenko,Vladimir, Chapurin.

[11]A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses. Zheng Jingyuan,Mao Zhenchuan,Xie Bingyan,Zheng Jingyuan,Zou Xuexiao.

[12]PeaT1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway. Yang, Xiufen,Qiu, Dewen,Guo, Lihua,Zeng, Hongmei,Mao, Jianjun,Gao, Qiufeng.

[13]Semi-dominant mutations in the CC-NB-LRR-type R gene, NLS1, lead to constitutive activation of defense responses in rice. Tang, Jiuyou,Wang, Yiqin,Liu, Linchuan,Xu, Bo,Li, Feng,Fang, Jun,Chu, Chengcai,Tang, Jiuyou,Wang, Yiqin,Liu, Linchuan,Xu, Bo,Li, Feng,Fang, Jun,Chu, Chengcai,Tang, Jiuyou,Liu, Linchuan,Xu, Bo,Li, Feng,Zhu, Xudong.

[14]Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings. Dong, Chun-Juan,Wang, Xiao-Li,Shang, Qing-Mao.

[15]The G protein-coupled receptors in the silkworm, Bombyx mori. Sun, Peng,Wang, Yu,Chen, Xin,Fan, Yi,He, Xiaobai,Deng, Xiaoyan,Chen, Xiaopan,Zhou, Naiming,Zhang, Guozheng.

[16]Georgenia halophila sp nov., a halophilic actinobacterium isolated from a salt lake. Tang, Shu-Kun,Li, Wen-Jun,Tang, Shu-Kun,Li, Wen-Jun,Wang, Yun,Lou, Kai,Lee, Jae-Chan,Park, Dong-Jin,Kim, Chang-Jin.

[17]A Novel Dehydration-Responsive Element-Binding Protein from Caragana korshinskii Is Involved in the Response to Multiple Abiotic Stresses and Enhances Stress Tolerance in Transgenic Tobacco. Wang, Xuemin,Dong, Jie,Gao, Hongwen,Liu, Yun.

[18]Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18. Tong, Tiegang,Bai, Yu,Liu, Guangliang,Wang, Qun,Zhang, Weijun,Xiao, Yihong,Xu, Shulan,Liu, Nihong,Yang, Tao,Wu, Donglai.

[19]Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine. Wang, Gui-Ping,Hui, Zhen,Li, Feng,Zhao, Mei-Rong,Zhang, Jin,Wang, Wei,Wang, Gui-Ping.

[20]Gene Expression of Heart and Adipocyte Fatty Acid-binding Protein in Chickens by FQ-RT-PCR. Tu, Yunjie,Su, Yijun,Wang, Kehua,Zhang, Xueyu,Tong, Haibing,Gao, Yushi.

作者其他论文 更多>>

微信小程序