Development of a UPLC-MS/MS-based method for simultaneous determination of advanced glycation end products and heterocyclic amines in stewed meat products

文献类型： 外文期刊

第一作者： Wang, Tan

作者： Wang, Tan;Zhao, Yueliang;Wang, Tan;Dong, Maofeng;Shen, Qinyi;Wen, Guangyue;Wang, Mingfu;Zhao, Yueliang;Wang, Mingfu

作者机构：

关键词： AGEs; HAs; UPLC-MS/MS; Stewed meat products

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 451 卷

页码：

收录情况： SCI

摘要： A novel analytical strategy was proposed to simultaneously quantify two advanced glycation end products (AGEs) including N-epsilon-(Carboxymethyl)lysine (CML), N-epsilon-(Carboxyethyl)lysine (CEL) and eight heterocyclic amines (HAs) including IQ, MeIQ, MeIQx, 4,8-DiMeIQx, 7,8-DiMeIQx, PhIP, Harman, and Norharman. The procedure was based on a two-step extraction, solid phase extraction (SPE) purification followed by ultra performance liquid chromatography tandem mass spectrometry. The established method showed a good linearity (R-2 >= 0.9950), rapid processing time (8 min per sample), satisfactory recoveries (matrix spiked recoveries range from 72.2% to 119.6%) and precision (intra-day and inter-day RSDs were <19.3%). The limit of quantification (LOQ) and limit of detection (LOD) resulted to be between 0.05-15 ng/g and 0.2-50 ng/g, respectively. The validated technique was further applied to determine HAs and AGEs in eight stewed meat product samples consumed in Shanghai, with the amount of HAs and AGEs ranging from 2.851 to 18.289 ng/g and 118.158-543.493 ng/g, respectively.

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