A rapid and high-throughput system for the detection of transgenic products based on LAMP-CRISPR-Cas12a

文献类型： 外文期刊

第一作者： Liu, Hua

作者： Liu, Hua;Zeng, Haijuan;He, Chuan;Wang, Jinbin;Cheng, Fang;Tang, Xueming;Tang, Xueming

作者机构：

关键词： GM organisms; CRISPR-Cas12a; LAMP; Rapid detection

期刊名称：CURRENT RESEARCH IN FOOD SCIENCE （ 影响因子：6.3； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 7 卷

页码：

收录情况： SCI

摘要： With the increasing acreage of genetically modified crops worldwide, rapid and efficient detection technologies have become very important for the regulation and screening of GM organisms. We constructed a method based on loop-mediated isothermal amplification (LAMP), CRISPR-Cas12a and lateral flow assay (LAMP-CRISPRCas12a-LFA). It is an intuitive, sensitive and specific fluorescence detection and test strip system to detect CP4EPSPS and Cry1Ab/Ac genes in field screening. The LAMP-CRISPR-Cas12a-LFA method has a limit of detection (LOD) of 100 copies based on lateral flow test strips after optimization of the conditions with screened specific primers, and the entire detection process can be completed within 1 h at 61 degrees C. The system was used to evaluate field test samples and showed high reproducibility after testing products containing CP4-EPSPS and Cry1Ab/Ac genes, and both were detectable. The LAMP-CRISPR-Cas12a-LFA method established in this paper functions as a rapid field detection method. It requires only one portable thermostatic instrument, which renders it compatible with the rapid detection of field samples and useable at experimental workstations, in law enforcement field work, and in local inspection and quarantine departments.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序