Comparative transcriptome analysis to identify the important mRNA and lncRNA associated with salinity tolerance in alfalfa

文献类型： 外文期刊

第一作者： Yang, Gaimei

作者： Yang, Gaimei;Rong, Mengru;Yu, Rugang;Zhang, Qiting;Du, Xueling;Xu, Xian;Li, Zhengyan;Xu, Zhiming;Wang, Guoliang

作者机构：

关键词： Medicago sativa; Comparative transcriptomics; Salinity stress; lncRNA; mRNA

期刊名称：PEERJ （ 影响因子：2.4； 五年影响因子：2.7 ）

ISSN： 2167-8359

年卷期： 2024 年 12 卷

页码：

收录情况： SCI

摘要： Salinity represents a fatal factor affecting the productivity of alfalfa. But the regulation of salinity tolerance via lncRNAs and mRNAs remains largely unclear within alfalfa. For evaluating salinity stress resistance-related lncRNAs and mRNAs within alfalfa, we analyzed root transcriptomics in two alfalfa varieties, GN5 (salinity-tolerant) and GN3 (salinity-sensitive), after treatments with NaCl at 0 and 150 mM. There were altogether 117,677 lncRNAs and 172,986 mRNAs detected, including 1,466 lncRNAs and 2,288 mRNAs with significant fi cant differential expression in GN5150/GN50, 150 /GN5 0 , GN3150/GN30, 150 /GN3 0 , GN50/GN30, 0 /GN3 0 , and GN5150/GN3150. 150 /GN3 150 . As revealed by GO as well as KEGG enrichment, some ionic and osmotic stress-associated genes, such as HPCA1-LRR, , PP2C60, , PP2C71, , CRK1, , APX3, , HXK2, , BAG6, , and ARF1, , had up-regulated levels in GN5 compared with in GN3. In addition, NaCl treatment markedly decreased CNGC1 expression in GN5. According to co-expressed network analyses, six lncRNAs (TCONS_00113549, TCONS_00399794, TCONS_00297228, TCONS_00004647, TCONS_00033214 and TCONS_00285177) modulated 66 genes including ARF1, , BAG6, , PP2C71, , and CNGC1 in alfalfa roots, suggesting that these nine genes and six lncRNAs probably facilitated the different salinity resistance in GN5 vs . GN3. These results shed more lights on molecular mechanisms underlying genotype difference in salinity tolerance among alfalfas.

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