Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs

文献类型： 外文期刊

第一作者： Li, Junliang

作者： Li, Junliang;Yu, Dawei;Du, Weihua;Zhao, Shanjiang;Pang, Yunwei;Hao, Haisheng;Zhao, Xueming;Zhu, Huabin;Zou, Huiying;Li, Junliang;Li, Shijie;Yu, Dawei;Wang, Jing;Li, Chongyang;Wang, Qingwei;Wang, Jing

作者机构：

关键词： pig; IG-DMR; DNA methylation; genomic imprinting; SCNT

期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.081； 五年影响因子：6.576 ）

ISSN： 2296-634X

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： Correct reprogramming of the DLK1-DIO3 imprinted region is critical for the development of cloned animals. However, in pigs, the imprinting and regulation of the DLK1-DIO3 region has not been systematically analyzed. The objective of this study was to investigate the imprinting status and methylation regulation of the DLK1-DIO3 region in wild-type and cloned neonatal pigs. We mapped the imprinting control region, IG-DMR, by homologous alignment and validated it in sperm, oocytes, fibroblasts, and parthenogenetic embryos. Subsequently, single nucleotide polymorphism-based sequencing and bisulfite sequencing polymerase chain reaction were conducted to analyze imprinting and methylation in different types of fibroblasts, as well as wild-type and cloned neonatal pigs. The results showed that Somatic cell nuclear transfer (SCNT) resulted in hypermethylation of the IG-DMR and aberrant gene expression in the DLK1-DIO3 region. Similar to wild-type pigs, imprinted expression and methylation were observed in the surviving cloned pigs, whereas in dead cloned pigs, the IG-DMR was hypermethylated and the expression of GTL2 was nearly undetectable. Our study reveals that abnormal imprinting of the DLK1-DIO3 region occurs in cloned pigs, which provides a theoretical basis for improving the cloning efficiency by gene editing to correct abnormal imprinting.

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