FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum

文献类型： 外文期刊

第一作者： Sun, Haiyan

作者： Sun, Haiyan;Cai, Shiyan;Li, Xinlei;Deng, Yuanyu;Yang, Xiaoyue;Cao, Shulin;Li, Wei;Chen, Huaigu;Sun, Haiyan;Chen, Huaigu;Liu, Huiquan

作者机构：

关键词： Fusarium graminearum; succinate dehydrogenasesubunitC; paralog; natural resistance; SDHI; molecular functions

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 51 期

页码：

收录情况： SCI

摘要： Fusarium graminearum exhibited natural resistance to a majority of succinate dehydrogenase inhibitor fungicides (SDHIs) and the molecular mechanisms responsible for the natural resistance were still unknown. Succinate dehydrogenase subunit C (SdhC) is an essential gene for maintaining succinate-ubiquinone oxidoreductase (SQR) function in fungi. In F. graminearum, a paralog of FgSdhC named as FgSdhC(1) was identified. Based on RNA-Seq and qRT-PCR assay, we found that the expression level of FgSdhC(1) was very low but upregulated by SDHIs treatment. Based on reverse genetics, we demonstrated that FgSdhC(1) was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard F. graminearum isolate PH-1 had high sensitivity to a majority of SDHIs. A single nucleotide variation (C to T) in the FgSdhC(1) of isolate PH-1, resulting in a premature termination codon (TAA) replacing the fourth amino acid glutamine (Q), led to the failure of FgSdhC(1) to perform functions of conferring nature resistance. These results established that a dispensable paralogous gene determined SDHIs resistance in natural populations of F. graminearum.

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