Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases

文献类型： 外文期刊

第一作者： Yin, Yuebang

作者： Yin, Yuebang;Yin, Yuebang;Liu, Peng-Yu;Liu, Peng-Yu;Shi, Yinghua;Li, Ping;Li, Ping;Li, Ping

作者机构：

关键词： Development; Disease; Organoids; Single-cell sequencing

期刊名称：REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY （ 影响因子：5.545； 五年影响因子：5.645 ）

ISSN： 0303-4240

年卷期： 2021 年 179 卷

页码：

收录情况： SCI

摘要： The development and function of a particular organ and the pathogenesis of various diseases remain intimately linked to the features of each cell type in the organ. Conventional messenger RNA- or protein-based methodologies often fail to elucidate the contribution of rare cell types, including some subpopulations of stem cells, short-lived progenitors and circulating tumour cells, thus hampering their applications in studies regarding organ development and diseases. The scRNA-seq technique represents a new approach for determining gene expression variability at the single-cell level. Organoids are new preclinical models that recapitulate complete or partial features of their original organ and are thought to be superior to cell models in mimicking the sophisticated spatiotemporal processes of the development and regeneration and diseases. In this review, we highlight recent advances in the field of scRNA-seq, organoids and their current applications and summarize the advantages of using a combination of scRNA-seq and organoid technology to model diseases and organ development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序