Vitrification cryo-foil method for shoot tip cryopreservation and virus eradication in apple

文献类型： 外文期刊

第一作者： Wang, Min-Rui

作者： Wang, Min-Rui;Bao, Jun-Hua;Ma, Xiao-Yan;Yan, Zi-Han;Zhang, Dong;Wang, Qiao-Chun;Wang, Min-Rui;Cui, Zhen-Hua;Zhu, Li-Ying

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关键词： Apple; Cryopreservation; Cryotherapy; Shoot tip; Virus eradication; V cryo-foil

期刊名称：CRYOBIOLOGY （ 影响因子：2.1； 五年影响因子：2.5 ）

ISSN： 0011-2240

年卷期： 2024 年 117 卷

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收录情况： SCI

摘要： Establishment of a new method for improved shoot tip cryopreservation is crucial to facilitate the long-term preservation of plant germplasm as well as the use of cryotherapy for pathogen eradication. The present study reported a vitrification (V) cryo-foil method for shoot tip cryopreservation and virus eradication in apple. Shoot tip regrowth levels after cryopreservation were comparable among V cryo-foil (53 %), V cryo-plate (46 %) and conventional droplet vitrification (Dr-vi, 48 %). The V cryo-foil is more efficient to perform than Dr-vi as more shoot tips can be cryopreserved by one person. In the histological study applying an image-overlaying strategy, shoot tips cryopreserved by V cryo-foil showed a higher survival chance in the youngest leaf primordia than in the apical dome. When V cryo-foil was tested for virus eradication, fifty-five percent (55 %) of cryo-derived shoots were free of the apple stem pitting virus (ASPV), while none and less than 10 % were free of the apple stem grooving virus (ASGV) and the apple chlorotic leaf spot virus (ACLSV), respectively. Thus, these two viruses were efficiently preserved by V cryo-foil cryopreservation. Noticeably, although the shoot regrowth level was reduced to 27 %, a higher frequency (81 %) of ASPV eradication was achieved when a reduced duration of cryoprotectant exposure was applied in V cryo-foil, supporting the use of insufficient cryoprotection for improved virus eradication.

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