Bombyx mori C-Type Lectin (BmIML-2) Inhibits the Proliferation of B. mori Nucleopolyhedrovirus (BmNPV) through Involvement in Apoptosis

文献类型： 外文期刊

第一作者： Mei, Xianghan

作者： Mei, Xianghan;Li, Chun;Peng, Peilin;Wang, Jue;He, Enxi;Qiu, Zhiyong;Xia, Dingguo;Zhao, Qiaoling;Shen, Dongxu;Mei, Xianghan;Li, Chun;Peng, Peilin;Wang, Jue;He, Enxi;Qiu, Zhiyong;Xia, Dingguo;Zhao, Qiaoling;Shen, Dongxu

作者机构：

关键词： Bombyx mori; C-type lectin; B; mori nucleopolyhedrovirus; apoptosis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

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年卷期： 2022 年 23 卷 15 期

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收录情况： SCI

摘要： C-type lectins (CTLs) are widely distributed in mammals, insects, and plants, which act as pattern recognition receptors (PRRs) to recognize pathogens and initiate immune responses. In this study, we identified a C-type lectin gene called BmIML-2 from the silkworm Bombyx mori. Its open reading frame (ORF) encodes 314 amino acids, which contain dual tandem C-type lectin-like domain (CTLD). BmIML-2 is highly expressed in the fat body and is significantly induced at 24 h after BmNPV infection. Moreover, overexpression of BmIML-2 dramatically inhibited the proliferation of BmNPV, and knockdown assay via siRNA further validated the inhibition of BmIML-2 on viral proliferation. In addition, transcript level detection of apoptosis-related genes and observation of apoptosis bodies implied that overexpression of BmIML-2 promoted BmNPV-induced apoptosis. Immunofluorescence analysis indicated that BmIML-2 distributed throughout the cytoplasm and was slightly concentrated in the cell membrane. Taken together, our results suggest that BmIML-2 could inhibit in the proliferation of BmNPV by facilitating cell apoptosis.

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