Study on molecular interaction between NifA and NifL of Pseudomonas stutzeri A1501 by using the yeast two-hybrid system

文献类型： 外文期刊

第一作者： Xie, ZH

作者： Xie, ZH;Yang, Y;Ping, SZ;Chen, M;Zhang, W;Lu, W;Xu, YQ;Liu, HJ;Wang, GY;Claudine, E;Lin, M

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期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Pseudomonas stutzeri A1501 (formerly Alcaligenes faecalis A1501), which was isolated from rice paddies in South China in 1980, can colonize tightly on rhizoplane of the host plants or invade the roots of plants for growth and nitrogen fixation. But A1501 can fix nitrogen only under the micro-aerobic and nitrogen-free conditions. The oxygen concentration and the availability of fixed nitrogen are therefore important factors in the regulation of nitrogenase biosynthesis of associative nitrogen-fixing bacteria. In the model diazotrophs Azotobacter vinelandii and Klebsiella pneumoniae, nitrogen fixation is controlled at the tran-scriptional level by the regulatory proteins encoded by nifLA. A 30 kb DNA region containing complete nifLA genes cluster (GenBank Accession No. AJ297529) from A1501 has been previously characterized. Genetic analysis showed that A1501 had similar nifLA operon to that in K. pneumoniae. Evidence suggests that in P. stutzeri A1501, NifA is the transcriptional activator and NifL acts as a negative regulator of nif genes in the presence of oxygen and ammonia (unpublished data). Inhibition of NifA activity by NifL requires stoichiometric amounts of two proteins, implying that the mechanism of inhibition is by direct protein-protein interaction rather than by catalytic modification of the NifA protein in the model diazotrophs A. vinelandii In this study, we used the yeast two-hybrid system to identify NifL-NifA protein-protein interaction in vivo.

分类号： N1

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