Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China

文献类型： 外文期刊

第一作者： Sun, YC

作者： Sun, YC;Chen, YC;Tian, ZX;Li, FM;Wang, XY;Zhang, J;Xiao, ZL;Lin, M;Gilmartin, N;Dowling, DN;Wang, YP

作者机构：

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN： 0099-2240

年卷期： 2005 年 71 卷 8 期

页码：

收录情况： SCI

摘要： Glyphosate has been used globally as a safe herbicide for weed control. It inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (AroA), which is a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. A Pseudomonas putida strain, 4G-1, was isolated from a soil heavily contaminated by glyphosate in China. Its AroA-encoding gene (aroA) has been cloned, sequenced, and expressed in Escherichia coli. Phylogenetic analysis revealed that this AroA belongs neither to class I nor to class 11 AroA enzymes. When compared with E. coli AroA, 4G-1 AroA shows similar values for K-m [PEP], K-m [S3P], and specific enzyme activity. Moreover, 4G-1 AroA exhibits high tolerance to glyphosate, which indicates a protein with a high potential for structural and functional studies of AroA in general and its potential usage for the generation of transgenic crops resistant to the herbicide.

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