Assessment of the role of an ABCC transporter TuMRP1 in the toxicity of abamectin to Tetranychus urticae

文献类型： 外文期刊

第一作者： Wu, Mingmei

作者： Wu, Mingmei;Zhang, Yan;Tian, Tian;Xu, Dandan;Wu, Qingjun;Xie, Wen;Zhang, Youjun;Guo, Zhaojiang;Wang, Shaoli;Tian, Tian;Xu, Dandan;Crickmore, Neil;Guo, Zhaojiang;Wang, Shaoli

作者机构：

关键词： Tetranychus urticae; Abamectin; Resistance mechanism; ABC transporter; TuMRP1

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 195 卷

页码：

收录情况： SCI

摘要： The rapid evolution of pest resistance threatens the sustainable utilization of bioinsecticides such as abamectin, and so deciphering the molecular mechanisms affecting toxicity and resistance is essential for their long-term application. Historical studies of abamectin resistance in arthropods have mainly focused on mechanisms involving the glutamate-gated chloride channel (GluCl) targets, with the role of metabolic processes less clear. The two-spotted spider mite, Tetranychus urticae, is a generalist herbivore notorious for rapidly developing resistance to pesticides worldwide, and abamectin has been widely used for its control in the field. After reanalyzing previous transcriptome and RNA-seq data, we here identified an ABC transporter subfamily C gene in T. urticae named multidrug resistance-associated protein 1 (TuMRP1), whose expression differed between susceptible and resistant populations. Synergism bioassays with the inhibitor MK-571, the existence of a genetic association between TuMRP1 expression and susceptibility to abamectin, and the effect of RNA interference mediated silencing of TuMRP1 were all consistent with a direct role of this transporter protein in the toxicity of abamectin. Although ABC transporters are often involved in removing insecticidal compounds from cells, our data suggest either an alternative role for these proteins in the mechanism of action of abamectin or highlight an indirect association between their expression and abamectin toxicity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序