Isolation of serotype 2 porcine teschovirus in China: Evidence of natural recombination

文献类型： 外文期刊

第一作者： Wang, Bin

作者： Wang, Bin;Tian, Zhi-Jun;Gong, Da-Qing;Li, Deng-Yun;Wang, Yao;Chen, Jia-Zeng;An, Tong-Qing;Peng, Jin-Mei;Wang, Bin;Tong, Guang-Zhi;Wang, Bin;Chen, Jia-Zeng;Peng, Jin-Mei;Tong, Guang-Zhi

作者机构：

关键词： polyprotein;GenBank sequence data;nucleotide sequence;phylogenetic tree;genetic recombination

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Porcine teschovirus (PTV), the pathogen of porcine polioencephalomyelitis, is a member of the family Picornaviridae. In this study, a new PTV strain (designated as JF613) was isolated from pigs in China. It was confirmed by the specific CPE on susceptible cells, RTPCR and nucleotide sequencing. Analysis of its amino acids sequence of complete polyprotein indicated that the isolate belongs to serotype 2. Genetic recombination is a well-known phenomenon for picornavirus which has been demonstrated in many other members of the family, but it remains so far unclear whether recombination occurs in PTV. To detect possible recombination events, 30 sequences of complete coding regions of PTV strains accessible in GenBank were examined. Putative recombinant sequence was identified with the use of SimPlot program. The result showed that the genomic sequence of our isolate exhibited highest similarities with strains of serotypes 2 and 5, respectively, in two crossover regions, suggesting the recombination event in PTV. Then the mosaic structure of viral genome was confirmed by bootscanning and genetic algorithm for recombination detection (GARD). This represents the first PTV-2 isolate in China. Furthermore, our study provided the first evidence of natural recombination in PTV and indicated that homologous recombination may be a driving force in PTV evolution

分类号： S8

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