Molecular signatures of in vitro produced embryos derived from ovum pick up or slaughterhouse oocytes in buffalo

文献类型： 外文期刊

第一作者： Yang, Chun-Yan

作者： Yang, Chun-Yan;Zheng, Hai-Ying;Abdelnour, Sameh A.;Li, Ling-yu;Shokrollahi, Borhan;Tang, Li-Ping;Zhang, Yu;Huang, Jia-Xiang;Shang, Jiang-Hua;Yang, Chun-Yan;Abdelnour, Sameh A.;Abdelnour, Sameh A.;Shokrollahi, Borhan;Zhang, Yu

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关键词： Buffalo; Abattoir ovaries; Ovum pick up; Development competence; Molecular mechanism

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2021 年 169 卷

页码：

收录情况： SCI

摘要： This study was performed to investigate the difference in developmental competence of oocytes derived from ovum pick-up (OPU) and slaughterhouse ovaries (SLH), and its underlying mechanisms. The OPU and SLH oocytes were in-vitro maturated and fertilized to produce blastocysts, and these blastoycsts were collected to explore the expression of key genes for developmental potential and telomere (Oct-4, Sox2, Nanog, Cdx2, Gata3, E-cadherin, b-catenin, TERT, TERF1 and TERF2). The results showed that both the cleavage and blastocyst rates were significantly higher for the OPU group (68.31%, 39.48%, respectively) than SLH group (57.59%, 26.50%, respectively) (P < 0.01). The relative mRNA abundances of Sox2, Oct-4, Nanog and E-cadherin were significantly higher in the OPU blastocysts than the SLH ones (P < 0.01). Protein expression analysis by Western blot and immunofluorescence also revealed that the expression of E-cadherin and Sox2 was significantly higher in OPU blastocysts than SLH ones. However, there was no significant differences between the two groups in the expression of Cdx2, b-catenin, Gata3, TERT, TERF1, TERF2. These results imply oocyte sources modify the expression of development and adhesion related genes in blastocysts, which may elucidate a possible reasoning for the low development competence of buffalo SLH embryos. (c) 2021 Elsevier Inc. All rights reserved.

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