The Breeding of High-Quality Dandelions by NaCl Induced Callus Variation Combined with a Drosophila Tumor Cell Migration Test

文献类型： 外文期刊

第一作者： Wu, Zhe

作者： Wu, Zhe;Li, Zhaojia;Feng, Wei;Meng, Ran;Wang, Xiuping;Wu, Chenxi

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关键词： cell migration; dandelion breeding; functional verification; salt-induced mutant; tissue culture

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

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年卷期： 2022 年 8 卷 12 期

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收录情况： SCI

摘要： Creating high-quality and salt-tolerant plant germplasm is an effective strategy to improve the utilization of saline-alkali land. Salt-induced callus mutation was used to create dandelion germplasm and mutant dandelion calluses were obtained under NaCl concentrations of 0.8%, 1%, and 1.2% with the identification of random amplified polymorphic DNA (RAPD) markers. A new dandelion line, "Binpu 2", selected from the progenies of dandelion tissue culture plantlets that originated from callus treated under 0.8% NaCl, was evaluated in light of its morphological characteristics, bioactive components, and antitumor functions. Results showed that the plant shape of "Binpu 2"was nearly upright; its cichoric acid content was 6.7 mg/g, which was 39.6% and 36.7% higher than its mother plant and local dandelion cultivar, respectively; its leaf water extracts of 0.2 g/mL had a significant inhibitory effect on cell polarity disruption-induced cell migration without affecting drosophila normal growth, revealed by the strong inhibitory effect on tumor cell migration, the increased level of MMP1 and beta-Integrin, and the reduced E-cadherin expression. Our results suggested that "Binpu 2" originated from salt-induced mutant performed better than its mother plant and processed strong antitumor function, which was suitable for amplified cultivation in saline-alkali land for food and medicinal industrial development.

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