Structural elucidation of a soluble polysaccharide XYLP-II from the fruiting body of Xylaria sp. L1 and its antibacterial and antioxidant activity

文献类型： 外文期刊

第一作者： Wang, Yitao

作者： Wang, Yitao;Shen, Nan;Wang, Zhihua;Yang, Shaohua;Cui, Kai

作者机构：

关键词： Polysaccharide; Structure; Antibacterial activities; Antioxidant activities

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 319 卷

页码：

收录情况： SCI

摘要： Xylaria can be used as food and medicine for its inflammatory and hepatoprotective effects. Of them, polysaccharide is a major bioactive ingredient. To explore the function of a novel polysaccharide (XYLP-II) purified from the fruiting body of Xylaria sp. L1, the structure, antibacterial and antioxidant activities, and its protective ability in the primary mouse jejunum mucosal epithelial cells of XYLP-II were extensively studied. The structural analysis of XYLP-II showed that monosaccharide composition of XYLP-II was only glucose, had a molecular weight of 345.43 kDa. Combined with FT-IR, methylation, and NMR analysis, it was preliminarily concluded that the main chain of XYLP-II was composed by -*4)-alpha-D-Glcp-(1 -* 2)-alpha-D-Glcp-(1-*, and the branch structure alpha-DGlcp-(1 -* 6)-alpha-D-Glcp-(1 -* connected to the O6 position of the main chain. Meanwhile, the antibacterial activity of XYLP-II on gram-positive bacteria was stronger and it increased cell permeability by destroying bacterial cell wall and cell membrane. In vitro experiments indicated XYLP-II effectively protected against oxidative damage in the mouse jejunum mucosal epithelial cells by increasing the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) and inhibiting the activity of malondialdehyde (MDA). Collectively, these results provide theoretical basis of Xylaria sp. L1 for its use in food additives and antioxidant therapeutics in medicine.

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