Investigating the Potential Mechanism of Methane Mitigation in Seaweed Gracilaria lemaneiformis via 16S rRNA Gene Sequencing and LC/MS-Based Metabolomics

文献类型： 外文期刊

第一作者： Sun, Yi

作者： Sun, Yi;Li, Shuai;Tong, Xiong;Zhang, Zhifei;Li, Dagang;Min, Li;Sun, Yi;Yang, Yufeng;Wang, Qing;Guo, Tongjun;Min, Li;Min, Li

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关键词： Gracilaria lemaneiformis; methane; rumen fermentation; microbial sequencing; metabolomics

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）

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年卷期： 2025 年 15 卷 16 期

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收录情况： SCI

摘要： Methane (CH4), originating from ruminants, is a major source of greenhouse gas emissions in the agriculture industry. This study aimed to determine the potential of red seaweed Gracilaria lemaneiformis (G. lemaneiformis) as an anti-methanogenic feed additive for cattle. Three supplementation levels of seaweed (2%, 5%, and 10% of dry matter) were evaluated for their effects on gas production and rumen fermentation characteristics during 48 h in vitro fermentation. The results revealed a significant decrease in total gas production (TGP), CO2, CH4, ammonia nitrogen (NH3-N), and volatile fatty acid (VFA) concentrations, with no differences in pH or dry matter disappearance (DMD). Notably, compared with the control group without seaweed, supplementation with 2% G. lemaneiformis effectively reduces CH4 emissions by 27.5% (p < 0.05). Supplementation with 2% G. lemaneiformis decreased the abundance of methanogens g_norank_f_Methanomethylophilaceae, responsible for CH4 generation, and increased the populations of bacteria (Kandleria and Succinivibrio) that compete with methanogens for substrates. Furthermore, upregulating the levels of 13(S)-HOTrE and 9(S)-HOTrE (polyunsaturated fatty acids) could inhibit methanogenic activity. Additionally, lower VFA concentrations will provide less raw materials for methane synthesis, thus further inhibiting methanogenesis. In summary, G. lemaneiformis, as a red seaweed with important economic value, can not only be applied to enhance marine carbon sinks but can also serve as a promising candidate for mitigating biomethane emissions in cattle.

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